8B6T

X-ray structure of the interface optimized haloalkane dehalogenase HaloTag7 fusion to the green fluorescent protein GFP (ChemoG5-TMR) labeled with a chloroalkane tetramethylrhodamine fluorophore substrate

Summary for 8B6T

Entry DOI	10.2210/pdb8b6t/pdb
Related	8B6R 8B6S
Descriptor	Green fluorescent protein,Haloalkane dehalogenase, [9-[2-carboxy-5-[2-[2-(6-chloranylhexoxy)ethoxy]ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethyl-azanium, CHLORIDE ION, ... (4 entities in total)
Functional Keywords	haloalkane dehalogenase, halotag, halotag7, self-labeling protein, green fluorescent protein, gfp, fluorophore, tetramethylerhodamine, tmr, hydrolase
Biological source	Rhodococcus sp. More
Total number of polymer chains	2
Total formula weight	121699.88
Authors	Tarnawski, M.,Hellweg, L.,Hiblot, J. (deposition date: 2022-09-27, release date: 2023-07-26, Last modification date: 2023-11-15)
Primary citation	Hellweg, L.,Edenhofer, A.,Barck, L.,Huppertz, M.C.,Frei, M.S.,Tarnawski, M.,Bergner, A.,Koch, B.,Johnsson, K.,Hiblot, J. A general method for the development of multicolor biosensors with large dynamic ranges. Nat.Chem.Biol., 19:1147-1157, 2023 Cited by PubMed: 37291200 DOI: 10.1038/s41589-023-01350-1 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2 Å)

Structure validation