8ARH

In situ subtomogram average of Vaccinia virus (WR) D13 lattice, on immature virions

Summary for 8ARH

• Entry DOI: 10.2210/pdb8arh/pdb
• EMDB information: 15602
• Descriptor: Scaffold protein D13 (1 entity in total)
• Functional Keywords: palisade, lattice, a4, virus
• Biological source: Vaccinia virus WR
• Total number of polymer chains: 6
• Total formula weight: 371691.19

Authors

Calcraft, T.,Hernandez-Gonzalez, M.,Nans, A.,Rosenthal, P.B.,Way, M. (deposition date: 2022-08-16, release date: 2023-02-01, Last modification date: 2024-07-24)

Primary citation

Hernandez-Gonzalez, M.,Calcraft, T.,Nans, A.,Rosenthal, P.B.,Way, M.
A succession of two viral lattices drives vaccinia virus assembly.
Plos Biol., 21:e3002005-e3002005, 2023

PubMed Abstract: During its cytoplasmic replication, vaccinia virus assembles non-infectious spherical immature virions (IV) coated by a viral D13 lattice. Subsequently, IV mature into infectious brick-shaped intracellular mature virions (IMV) that lack D13. Here, we performed cryo-electron tomography (cryo-ET) of frozen-hydrated vaccinia-infected cells to structurally characterise the maturation process in situ. During IMV formation, a new viral core forms inside IV with a wall consisting of trimeric pillars arranged in a new pseudohexagonal lattice. This lattice appears as a palisade in cross-section. As maturation occurs, which involves a 50% reduction in particle volume, the viral membrane becomes corrugated as it adapts to the newly formed viral core in a process that does not appear to require membrane removal. Our study suggests that the length of this core is determined by the D13 lattice and that the consecutive D13 and palisade lattices control virion shape and dimensions during vaccinia assembly and maturation.

PubMed: 36862727
DOI: 10.1371/journal.pbio.3002005

Experimental method

ELECTRON MICROSCOPY (19.2 Å)