8ARH
In situ subtomogram average of Vaccinia virus (WR) D13 lattice, on immature virions
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SUBTOMOGRAM AVERAGING |
| Resolution (Å) | 19.2 |
| Software | RELION,FEI tomography,CTFFIND,NOVACTF,UCSF ChimeraX |
| Symmetry | C6 |
| CTF correction | PHASE FLIPPING ONLY |
