8ANY
Human mitochondrial ribosome in complex with LRPPRC, SLIRP, A-site, P-site, E-site tRNAs and mRNA
This is a non-PDB format compatible entry.
Summary for 8ANY
| Entry DOI | 10.2210/pdb8any/pdb |
| EMDB information | 15544 16407 16408 16409 16410 16411 16412 16413 16414 |
| Descriptor | 12S mitochondrial rRNA, 28S ribosomal protein S12, mitochondrial, GUANOSINE-5'-DIPHOSPHATE, ... (103 entities in total) |
| Functional Keywords | mitochondrial translation, mrna delivery, ribosome |
| Biological source | Homo sapiens (human) More |
| Total number of polymer chains | 96 |
| Total formula weight | 3309204.95 |
| Authors | Singh, V.,Itoh, Y.,Amunts, A. (deposition date: 2022-08-06, release date: 2023-08-16, Last modification date: 2024-09-04) |
| Primary citation | Singh, V.,Moran, J.C.,Itoh, Y.,Soto, I.C.,Fontanesi, F.,Couvillion, M.,Huynen, M.A.,Churchman, L.S.,Barrientos, A.,Amunts, A. Structural basis of LRPPRC-SLIRP-dependent translation by the mitoribosome. Nat.Struct.Mol.Biol., 2024 Cited by PubMed Abstract: In mammalian mitochondria, mRNAs are cotranscriptionally stabilized by the protein factor LRPPRC (leucine-rich pentatricopeptide repeat-containing protein). Here, we characterize LRPPRC as an mRNA delivery factor and report its cryo-electron microscopy structure in complex with SLIRP (SRA stem-loop-interacting RNA-binding protein), mRNA and the mitoribosome. The structure shows that LRPPRC associates with the mitoribosomal proteins mS39 and the N terminus of mS31 through recognition of the LRPPRC helical repeats. Together, the proteins form a corridor for handoff of the mRNA. The mRNA is directly bound to SLIRP, which also has a stabilizing function for LRPPRC. To delineate the effect of LRPPRC on individual mitochondrial transcripts, we used RNA sequencing, metabolic labeling and mitoribosome profiling, which showed a transcript-specific influence on mRNA translation efficiency, with cytochrome c oxidase subunit 1 and 2 translation being the most affected. Our data suggest that LRPPRC-SLIRP acts in recruitment of mitochondrial mRNAs to modulate their translation. Collectively, the data define LRPPRC-SLIRP as a regulator of the mitochondrial gene expression system. PubMed: 39134711DOI: 10.1038/s41594-024-01365-9 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.85 Å) |
Structure validation
Download full validation report
