8AES

Crystal structure of a thermophilic O6-alkylguanine-DNA alkyltransferase-derived self-labeling protein-tag

Summary for 8AES

Entry DOI	10.2210/pdb8aes/pdb
Descriptor	Methylated-DNA--protein-cysteine methyltransferase (2 entities in total)
Functional Keywords	self-labelling protein tag, rational mutagenesis, o6-alkylguanine-dna alkyltransferase, clip-tag., transferase
Biological source	Saccharolobus solfataricus
Total number of polymer chains	10
Total formula weight	190491.76
Authors	Genta, M.,Perugino, G.,Miggiano, R. (deposition date: 2022-07-13, release date: 2022-10-12, Last modification date: 2024-03-27)
Primary citation	Merlo, R.,Mattossovich, R.,Genta, M.,Valenti, A.,Di Mauro, G.,Minassi, A.,Miggiano, R.,Perugino, G. First thermostable CLIP- tag by rational design applied to an archaeal O 6 -alkyl-guanine-DNA-alkyl-transferase. Comput Struct Biotechnol J, 20:5275-5286, 2022 Cited by PubMed: 36212535 DOI: 10.1016/j.csbj.2022.09.015 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.8 Å)

Structure validation