8AAN

The nucleoprotein complex of Rep protein with iteron containing dsDNA and DUE ssDNA.

Summary for 8AAN

• Entry DOI: 10.2210/pdb8aan/pdb
• Descriptor: DNA (5'-D(*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*AP*GP*T)-3'), DNA (5'-D(*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*GP*GP*T)-3'), Replication initiation protein, ... (6 entities in total)
• Functional Keywords: replication initiation protein nucleoprotein complex nucleic acid binding dna binding, dna binding protein
• Biological source: Escherichia coli; More
• Total number of polymer chains: 4
• Total formula weight: 45269.17

Authors

Nowacka, M.,Wegrzyn, K. (deposition date: 2022-07-01, release date: 2023-09-27, Last modification date: 2023-11-08)

• Primary citation: Wegrzyn, K.,Oliwa, M.,Nowacka, M.,Zabrocka, E.,Bury, K.,Purzycki, P.,Czaplewska, P.,Pipka, J.,Giraldo, R.,Konieczny, I.; Rep protein accommodates together dsDNA and ssDNA which enables a loop-back mechanism to plasmid DNA replication initiation.; Nucleic Acids Res., 51:10551-10567, 2023; PubMed: 37713613; DOI: 10.1093/nar/gkad740

Experimental method

X-RAY DIFFRACTION (2.19 Å)