8AAN

The nucleoprotein complex of Rep protein with iteron containing dsDNA and DUE ssDNA.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979310
Spacegroup name	C 1 2 1
Unit cell lengths	109.313, 81.047, 75.833
Unit cell angles	90.00, 123.52, 90.00

Refinement procedure

Resolution	29.940 - 2.190
R-factor	0.1887
Rwork	0.186
R-free	0.23290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rep
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.940	2.267
High resolution limit [Å]	2.189	2.189
Number of reflections	27949	4267
<I/σ(I)>	11.5
Completeness [%]	97.6
Redundancy	6.615
CC(1/2)	0.995	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	10% w/v PEG 20 000, 20% v/v PEG MME 550 0.02M of each carboxylic acid (0.2 M sodium formate, 0.2M ammonium acetate, 0.2M trisodium citrate, 0.2M sodium potassium L-tartrate, 0.2M sodium oxamate), 0.1M MES/imidazole pH 6.5.