8UUQ
Structure of the Measles virus Fusion protein in the pre-fusion conformation with bound [FIP-HRC]2-PEG11
Summary for 8UUQ
| Entry DOI | 10.2210/pdb8uuq/pdb |
| EMDB information | 42595 |
| Related PRD ID | PRD_002539 |
| Descriptor | Fusion glycoprotein F0, [FIP-HRC]2-PEG11, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total) |
| Functional Keywords | viral protein, glycoprotein, immune system, measles, high-resolution, ectodomain, post-fusion, small molecule, inhibitor, complex |
| Biological source | Measles virus strain Ichinose-B95a More |
| Total number of polymer chains | 9 |
| Total formula weight | 178534.84 |
| Authors | Zyla, D.,Saphire, E.O. (deposition date: 2023-11-01, release date: 2024-07-03, Last modification date: 2024-11-13) |
| Primary citation | Zyla, D.S.,Della Marca, R.,Niemeyer, G.,Zipursky, G.,Stearns, K.,Leedale, C.,Sobolik, E.B.,Callaway, H.M.,Hariharan, C.,Peng, W.,Parekh, D.,Marcink, T.C.,Diaz Avalos, R.,Horvat, B.,Mathieu, C.,Snijder, J.,Greninger, A.L.,Hastie, K.M.,Niewiesk, S.,Moscona, A.,Porotto, M.,Ollmann Saphire, E. A neutralizing antibody prevents postfusion transition of measles virus fusion protein. Science, 384:eadm8693-eadm8693, 2024 Cited by PubMed Abstract: Measles virus (MeV) presents a public health threat that is escalating as vaccine coverage in the general population declines and as populations of immunocompromised individuals, who cannot be vaccinated, increase. There are no approved therapeutics for MeV. Neutralizing antibodies targeting viral fusion are one potential therapeutic approach but have not yet been structurally characterized or advanced to clinical use. We present cryo-electron microscopy (cryo-EM) structures of prefusion F alone [2.1-angstrom (Å) resolution], F complexed with a fusion-inhibitory peptide (2.3-Å resolution), F complexed with the neutralizing and protective monoclonal antibody (mAb) 77 (2.6-Å resolution), and an additional structure of postfusion F (2.7-Å resolution). In vitro assays and examination of additional EM classes show that mAb 77 binds prefusion F, arrests F in an intermediate state, and prevents transition to the postfusion conformation. These structures shed light on antibody-mediated neutralization that involves arrest of fusion proteins in an intermediate state. PubMed: 38935733DOI: 10.1126/science.adm8693 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.34 Å) |
Structure validation
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