8D0X
Human FUT9 bound to LNnT
Summary for 8D0X
| Entry DOI | 10.2210/pdb8d0x/pdb |
| Related | 8D0O 8D0P 8D0Q 8D0R 8D0S 8D0U 8D0W |
| Descriptor | 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase 9, alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-beta-D-galactopyranose, ... (7 entities in total) |
| Functional Keywords | glycosyltransferase, alpha-(1, 3)-fucosyltransferase, gt 10, gt-b fold, inverting mechanism, transferase |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 1 |
| Total formula weight | 39871.42 |
| Authors | Kadirvelraj, R.,Wood, Z.A. (deposition date: 2022-05-26, release date: 2023-05-24, Last modification date: 2024-10-30) |
| Primary citation | Kadirvelraj, R.,Boruah, B.M.,Wang, S.,Chapla, D.,Huang, C.,Ramiah, A.,Hudson, K.L.,Prudden, A.R.,Boons, G.J.,Withers, S.G.,Wood, Z.A.,Moremen, K.W. Structural basis for Lewis antigen synthesis by the alpha 1,3-fucosyltransferase FUT9. Nat.Chem.Biol., 19:1022-1030, 2023 Cited by PubMed Abstract: Mammalian cell surface and secreted glycoproteins exhibit remarkable glycan structural diversity that contributes to numerous physiological and pathogenic interactions. Terminal glycan structures include Lewis antigens synthesized by a collection of α1,3/4-fucosyltransferases (CAZy GT10 family). At present, the only available crystallographic structure of a GT10 member is that of the Helicobacter pylori α1,3-fucosyltransferase, but mammalian GT10 fucosyltransferases are distinct in sequence and substrate specificity compared with the bacterial enzyme. Here, we determined crystal structures of human FUT9, an α1,3-fucosyltransferase that generates Lewis and Lewis antigens, in complex with GDP, acceptor glycans, and as a FUT9-donor analog-acceptor Michaelis complex. The structures reveal substrate specificity determinants and allow prediction of a catalytic model supported by kinetic analyses of numerous active site mutants. Comparisons with other GT10 fucosyltransferases and GT-B fold glycosyltransferases provide evidence for modular evolution of donor- and acceptor-binding sites and specificity for Lewis antigen synthesis among mammalian GT10 fucosyltransferases. PubMed: 37202521DOI: 10.1038/s41589-023-01345-y PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.33 Å) |
Structure validation
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