7ZXU

SARS-CoV-2 Omicron BA.4/5 RBD in complex with Beta-27 Fab and C1 nanobody

Summary for 7ZXU

• Entry DOI: 10.2210/pdb7zxu/pdb
• Descriptor: Spike protein S1, Nanobody C1, Beta-27 heavy chain, ... (9 entities in total)
• Functional Keywords: sars-cov-2, omicron ba.4, ba.5, rbd, antibody, fab, beta-27, viral protein/immune system, viral protein
• Biological source: Severe acute respiratory syndrome coronavirus 2; More
• Total number of polymer chains: 4
• Total formula weight: 86015.01

Authors

Huo, J.,Zhou, D.,Ren, J.,Stuart, D.I. (deposition date: 2022-05-23, release date: 2022-06-29, Last modification date: 2024-01-31)

Primary citation

Tuekprakhon, A.,Nutalai, R.,Dijokaite-Guraliuc, A.,Zhou, D.,Ginn, H.M.,Selvaraj, M.,Liu, C.,Mentzer, A.J.,Supasa, P.,Duyvesteyn, H.M.E.,Das, R.,Skelly, D.,Ritter, T.G.,Amini, A.,Bibi, S.,Adele, S.,Johnson, S.A.,Constantinides, B.,Webster, H.,Temperton, N.,Klenerman, P.,Barnes, E.,Dunachie, S.J.,Crook, D.,Pollard, A.J.,Lambe, T.,Goulder, P.,Paterson, N.G.,Williams, M.A.,Hall, D.R.,Fry, E.E.,Huo, J.,Mongkolsapaya, J.,Ren, J.,Stuart, D.I.,Screaton, G.R.
Antibody escape of SARS-CoV-2 Omicron BA.4 and BA.5 from vaccine and BA.1 serum.
Cell, 185:2422-, 2022

PubMed Abstract: The Omicron lineage of SARS-CoV-2, which was first described in November 2021, spread rapidly to become globally dominant and has split into a number of sublineages. BA.1 dominated the initial wave but has been replaced by BA.2 in many countries. Recent sequencing from South Africa's Gauteng region uncovered two new sublineages, BA.4 and BA.5, which are taking over locally, driving a new wave. BA.4 and BA.5 contain identical spike sequences, and although closely related to BA.2, they contain further mutations in the receptor-binding domain of their spikes. Here, we study the neutralization of BA.4/5 using a range of vaccine and naturally immune serum and panels of monoclonal antibodies. BA.4/5 shows reduced neutralization by the serum from individuals vaccinated with triple doses of AstraZeneca or Pfizer vaccine compared with BA.1 and BA.2. Furthermore, using the serum from BA.1 vaccine breakthrough infections, there are, likewise, significant reductions in the neutralization of BA.4/5, raising the possibility of repeat Omicron infections.

PubMed: 35772405
DOI: 10.1016/j.cell.2022.06.005

Experimental method

X-RAY DIFFRACTION (1.89 Å)