7ZXE

Structure of SNAPc containing Pol II pre-initiation complex bound to U1 snRNA promoter (OC)

Summary for 7ZXE

• Entry DOI: 10.2210/pdb7zxe/pdb
• EMDB information: 15009
• Descriptor: Transcription initiation factor IIB, snRNA-activating protein complex subunit 5, ZINC ION, ... (11 entities in total)
• Functional Keywords: rna polymerase ii, pol ii, pic, snapc, snrna, u1 promoter, transcription
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 10
• Total formula weight: 446858.88

Authors

Rengachari, S.,Schilbach, S.,Kaliyappan, T.,Gouge, J.,Zumer, K.,Schwarz, J.,Urlaub, H.,Dienemann, C.,Vannini, A.,Cramer, P. (deposition date: 2022-05-20, release date: 2022-11-30, Last modification date: 2024-07-24)

Primary citation

Rengachari, S.,Schilbach, S.,Kaliyappan, T.,Gouge, J.,Zumer, K.,Schwarz, J.,Urlaub, H.,Dienemann, C.,Vannini, A.,Cramer, P.
Structural basis of SNAPc-dependent snRNA transcription initiation by RNA polymerase II.
Nat.Struct.Mol.Biol., 29:1159-1169, 2022

PubMed Abstract: RNA polymerase II (Pol II) carries out transcription of both protein-coding and non-coding genes. Whereas Pol II initiation at protein-coding genes has been studied in detail, Pol II initiation at non-coding genes, such as small nuclear RNA (snRNA) genes, is less well understood at the structural level. Here, we study Pol II initiation at snRNA gene promoters and show that the snRNA-activating protein complex (SNAPc) enables DNA opening and transcription initiation independent of TFIIE and TFIIH in vitro. We then resolve cryo-EM structures of the SNAPc-containing Pol IIpre-initiation complex (PIC) assembled on U1 and U5 snRNA promoters. The core of SNAPc binds two turns of DNA and recognizes the snRNA promoter-specific proximal sequence element (PSE), located upstream of the TATA box-binding protein TBP. Two extensions of SNAPc, called wing-1 and wing-2, bind TFIIA and TFIIB, respectively, explaining how SNAPc directs Pol II to snRNA promoters. Comparison of structures of closed and open promoter complexes elucidates TFIIH-independent DNA opening. These results provide the structural basis of Pol II initiation at non-coding RNA gene promoters.

PubMed: 36424526
DOI: 10.1038/s41594-022-00857-w

Experimental method

ELECTRON MICROSCOPY (3.5 Å)