7ZOY
Crystal structure of Synechocystis halorhodopsin (SyHR), SO4-bound form, ground state
Summary for 7ZOY
| Entry DOI | 10.2210/pdb7zoy/pdb |
| Descriptor | Synechocystis halorhodopsin, CHLORIDE ION, SULFATE ION, ... (7 entities in total) |
| Functional Keywords | rhodopsin, syhr, trapping, cryo, cryotrapping, o-state, k-state, sulfate, chloride, pump, anion, photocycle, ion transport, membrane protein |
| Biological source | Synechocystis sp. PCC 7509 |
| Total number of polymer chains | 1 |
| Total formula weight | 30924.69 |
| Authors | Kovalev, K.,Bukhdruker, S.,Astashkin, R.,Vaganova, S.,Gordeliy, V. (deposition date: 2022-04-26, release date: 2022-11-09, Last modification date: 2024-01-31) |
| Primary citation | Astashkin, R.,Kovalev, K.,Bukhdruker, S.,Vaganova, S.,Kuzmin, A.,Alekseev, A.,Balandin, T.,Zabelskii, D.,Gushchin, I.,Royant, A.,Volkov, D.,Bourenkov, G.,Koonin, E.,Engelhard, M.,Bamberg, E.,Gordeliy, V. Structural insights into light-driven anion pumping in cyanobacteria. Nat Commun, 13:6460-6460, 2022 Cited by PubMed Abstract: Transmembrane ion transport is a key process in living cells. Active transport of ions is carried out by various ion transporters including microbial rhodopsins (MRs). MRs perform diverse functions such as active and passive ion transport, photo-sensing, and others. In particular, MRs can pump various monovalent ions like Na, K, Cl, I, NO. The only characterized MR proposed to pump sulfate in addition to halides belongs to the cyanobacterium Synechocystis sp. PCC 7509 and is named Synechocystis halorhodopsin (SyHR). The structural study of SyHR may help to understand what makes an MR pump divalent ions. Here we present the crystal structure of SyHR in the ground state, the structure of its sulfate-bound form as well as two photoreaction intermediates, the K and O states. These data reveal the molecular origin of the unique properties of the protein (exceptionally strong chloride binding and proposed pumping of divalent anions) and sheds light on the mechanism of anion release and uptake in cyanobacterial halorhodopsins. The unique properties of SyHR highlight its potential as an optogenetics tool and may help engineer different types of anion pumps with applications in optogenetics. PubMed: 36309497DOI: 10.1038/s41467-022-34019-9 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.91 Å) |
Structure validation
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