7ZOY
Crystal structure of Synechocystis halorhodopsin (SyHR), SO4-bound form, ground state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 62.300, 62.300, 110.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.270 - 1.910 |
| R-factor | 0.1964 |
| Rwork | 0.195 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hyj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.518 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.450 | 1.940 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rpim | 0.081 | 0.816 |
| Number of reflections | 19823 | 992 |
| <I/σ(I)> | 7.5 | 0.9 |
| Completeness [%] | 99.6 | |
| Redundancy | 10.7 | |
| CC(1/2) | 0.997 | 0.399 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7 | 293 | 2.0 M Ammonium Sulfate, 0.1 M HEPES |
