7ZOY
Crystal structure of Synechocystis halorhodopsin (SyHR), SO4-bound form, ground state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 62.300, 62.300, 110.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.270 - 1.910 |
R-factor | 0.1964 |
Rwork | 0.195 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hyj |
RMSD bond length | 0.011 |
RMSD bond angle | 1.518 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.450 | 1.940 |
High resolution limit [Å] | 1.910 | 1.910 |
Rpim | 0.081 | 0.816 |
Number of reflections | 19823 | 992 |
<I/σ(I)> | 7.5 | 0.9 |
Completeness [%] | 99.6 | |
Redundancy | 10.7 | |
CC(1/2) | 0.997 | 0.399 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7 | 293 | 2.0 M Ammonium Sulfate, 0.1 M HEPES |