7ZCT

Structure of the red fluorescent protein mScarlet3 at pH 7.5

Summary for 7ZCT

• Entry DOI: 10.2210/pdb7zct/pdb
• Descriptor: Red fluorescent protein drFP583 (2 entities in total)
• Functional Keywords: red fluorescent protein, high fluorescence quantum yield, efficient chromophore maturation, fluorescent protein
• Biological source: Discosoma sp.
• Total number of polymer chains: 2
• Total formula weight: 51730.26

Authors

Aumonier, S.,Dupuy, J.,Royant, A. (deposition date: 2022-03-28, release date: 2023-04-05, Last modification date: 2024-02-07)

Primary citation

Gadella Jr., T.W.J.,van Weeren, L.,Stouthamer, J.,Hink, M.A.,Wolters, A.H.G.,Giepmans, B.N.G.,Aumonier, S.,Dupuy, J.,Royant, A.
mScarlet3: a brilliant and fast-maturing red fluorescent protein.
Nat.Methods, 20:541-545, 2023

PubMed Abstract: We report the evolution of mScarlet3, a cysteine-free monomeric red fluorescent protein with fast and complete maturation, as well as record brightness, quantum yield (75%) and fluorescence lifetime (4.0 ns). The mScarlet3 crystal structure reveals a barrel rigidified at one of its heads by a large hydrophobic patch of internal residues. mScarlet3 behaves well as a fusion tag, displays no apparent cytotoxicity and it surpasses existing red fluorescent proteins as a Förster resonance energy transfer acceptor and as a reporter in transient expression systems.

PubMed: 36973546
DOI: 10.1038/s41592-023-01809-y

Experimental method

X-RAY DIFFRACTION (1.33 Å)