7ZCT

Structure of the red fluorescent protein mScarlet3 at pH 7.5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Red fluorescent protein drFP583	polymer	229	25865.1	2	UniProt (Q9U6Y8) Pfam (PF01353)	Discosoma sp.	DsRed
2	C, D (A, B)	water	water		18.0	244	Chemie (HOH)

Sequence modifications

A, B: 8 - 222 (UniProt: Q9U6Y8)

PDB	External Database	Details
Met 2	-	initiating methionine
Asp 3	-	expression tag
Ser 4	-	expression tag
Thr 5	-	expression tag
Glu 6	-	expression tag
Ala 7	-	expression tag
His 18	Arg 17	engineered mutation
Ser 22	Thr 21	engineered mutation
Met 23	Val 22	engineered mutation
Thr 42	His 41	engineered mutation
Gln 43	Asn 42	engineered mutation
Ala 45	Val 44	engineered mutation
Arg 48	Lys 47	engineered mutation
Ser 58	Ala 57	engineered mutation
Nrq 67	Gln 66	chromophore
-	Tyr 67	chromophore
-	Gly 68	chromophore
Arg 71	Lys 70	engineered mutation
Ala 72	Val 71	engineered mutation
Phe 73	Tyr 72	engineered mutation
Thr 74	Val 73	engineered mutation
Trp 84	Lys 83	engineered mutation
Gln 86	Leu 85	engineered mutation
Ala 105	Val 104	engineered mutation
Ser 107	Thr 106	engineered mutation
Ala 109	Thr 108	engineered mutation
Thr 112	Ser 111	engineered mutation
Glu 115	Gln 114	engineered mutation
Thr 118	Cys 117	engineered mutation
Leu 119	Phe 118	engineered mutation
Leu 125	Phe 124	engineered mutation
Arg 126	Ile 125	engineered mutation
Thr 128	Val 127	engineered mutation
Pro 132	Ser 131	engineered mutation
Glu 154	Arg 153	engineered mutation
Val 156	Gly 155	engineered mutation
Asp 161	Glu 160	engineered mutation
Lys 163	His 162	engineered mutation
Met 164	Lys 163	engineered mutation
Arg 167	Lys 166	engineered mutation
Arg 173	His 172	engineered mutation
Ala 176	Val 175	engineered mutation
Asp 177	Glu 176	engineered mutation
Thr 180	Ser 179	engineered mutation
Thr 181	Ile 180	engineered mutation
Arg 183	Met 182	engineered mutation
Met 190	Leu 189	engineered mutation
Ala 193	Tyr 192	engineered mutation
Phe 194	Tyr 193	engineered mutation
Asn 195	Tyr 194	engineered mutation
Ile 196	Val 195	engineered mutation
Arg 198	Ser 197	engineered mutation
Val 211	Ile 210	engineered mutation
Ser 218	Thr 217	engineered mutation
Val 219	Glu 218	engineered mutation
Ala 220	Gly 219	engineered mutation
Ser 223	-	expression tag
Thr 224	-	expression tag
Gly 225	-	expression tag
Gly 226	-	expression tag
Ser 227	-	expression tag
Gly 228	-	expression tag
Gly 229	-	expression tag
Ser 230	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51730.3
All*	Total formula weight	51730.3

*Water molecules are not included.