7Z0O

Structure of transcription factor UAF in complex with TBP and 35S rRNA promoter DNA

Summary for 7Z0O

• Entry DOI: 10.2210/pdb7z0o/pdb
• EMDB information: 14428
• Descriptor: Histone H3, Histone H4, RNA polymerase I-specific transcription initiation factor RRN5, ... (9 entities in total)
• Functional Keywords: transcription factor, nucleolus, histone fold, transcription
• Biological source: Saccharomyces cerevisiae (baker's yeast); More
• Total number of polymer chains: 10
• Total formula weight: 289982.72

Authors

Baudin, F.,Murciano, B.,Fung, H.K.H.,Fromm, S.A.,Mueller, C.W. (deposition date: 2022-02-23, release date: 2022-04-27, Last modification date: 2024-07-17)

Primary citation

Baudin, F.,Murciano, B.,Fung, H.K.H.,Fromm, S.A.,Mattei, S.,Mahamid, J.,Muller, C.W.
Mechanism of RNA polymerase I selection by transcription factor UAF.
Sci Adv, 8:eabn5725-eabn5725, 2022

PubMed Abstract: Preribosomal RNA is selectively transcribed by RNA polymerase (Pol) I in eukaryotes. The yeast transcription factor upstream activating factor (UAF) represses Pol II transcription and mediates Pol I preinitiation complex (PIC) formation at the 35 ribosomal RNA gene. To visualize the molecular intermediates toward PIC formation, we determined the structure of UAF in complex with native promoter DNA and transcription factor TATA-box-binding protein (TBP). We found that UAF recognizes DNA using a hexameric histone-like scaffold with markedly different interactions compared with the nucleosome and the histone-fold-rich transcription factor IID (TFIID). In parallel, UAF positions TBP for Core Factor binding, which leads to Pol I recruitment, while sequestering it from DNA and Pol II/III-specific transcription factors. Our work thus reveals the structural basis of RNA Pol selection by a transcription factor.

PubMed: 35442737
DOI: 10.1126/sciadv.abn5725

Experimental method

ELECTRON MICROSCOPY (2.8 Å)