7YVZ

Structure of Caenorhabditis elegans CISD-1/mitoNEET

Summary for 7YVZ

• Entry DOI: 10.2210/pdb7yvz/pdb
• Descriptor: CDGSH iron-sulfur domain-containing protein 1 (CISD-1/mitoNEET), FE2/S2 (INORGANIC) CLUSTER (3 entities in total)
• Functional Keywords: caenorhabditis elegans, mitochondrial outer membrane protein, cdgsh iron sulfur containing protein, regulating mitochondrial function and morphology, metal binding protein
• Biological source: Caenorhabditis elegans
• Total number of polymer chains: 1
• Total formula weight: 9806.78

Authors

Hasegawa, K.,Hagiuda, E.,Taguchi, A.T.,Geldenhuys, W.,Iwasaki, T.,Kumasaka, T. (deposition date: 2022-08-20, release date: 2022-10-19, Last modification date: 2024-10-09)

Primary citation

Boos, J.R.,Jandrain, H.N.,Hagiuda, E.,Taguchi, A.T.,Hasegawa, K.,Fedun, B.L.,Taylor, S.J.,Elad, S.M.,Faber, S.E.,Kumasaka, T.,Iwasaki, T.,Geldenhuys, W.J.
Structure and biological evaluation of Caenorhabditis elegans CISD-1/mitoNEET, a KLP-17 tail domain homologue, supports attenuation of paraquat-induced oxidative stress through a p38 MAPK-mediated antioxidant defense response.
Adv Redox Res, 6:-, 2022

PubMed Abstract: CISD-1/mitoNEET is an evolutionarily conserved outer mitochondrial membrane [2Fe-2S] protein that regulates mitochondrial function and morphology. The [2Fe-2S] clusters are redox reactive and shown to mediate oxidative stress and . However, there is limited research studying CISD-1/mitoNEET mediation of oxidative stress in response to environmental stressors. In this study, we have determined the X-ray crystal structure of CISD-1/mitoNEET homologue and evaluated the mechanisms of oxidative stress resistance to the pro-oxidant paraquat in age-synchronized populations by generating gain and loss of function CISD-1 models. The structure of the CISD-1/mitoNEET soluble domain refined at 1.70-Å resolution uniquely shows a reversible disulfide linkage at the homo-dimeric interface and also represents the N-terminal tail domain for dimerization of the cognate kinesin motor protein KLP-17 involved in chromosome segregation dynamics and germline development of the nematode. Moreover, overexpression of CISD-1/mitoNEET in has revealed beneficial effects on oxidative stress resistance against paraquat-induced reactive oxygen species generation, corroborated by increased activation of the p38 mitogen-activated protein kinase (MAPK) signaling cascade.

PubMed: 36533211
DOI: 10.1016/j.arres.2022.100048

Experimental method

X-RAY DIFFRACTION (1.7 Å)