7YVZ
Structure of Caenorhabditis elegans CISD-1/mitoNEET
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-08 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 37.798, 37.798, 82.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.350 - 1.700 |
R-factor | 0.1699 |
Rwork | 0.168 |
R-free | 0.18910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | rat mitoNEET |
RMSD bond length | 0.015 |
RMSD bond angle | 1.461 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.1192.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.350 | 1.761 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.049 | 0.472 |
Rmeas | 0.053 | 0.508 |
Rpim | 0.020 | 0.185 |
Number of reflections | 7076 | 686 |
<I/σ(I)> | 19.83 | 3.5 |
Completeness [%] | 99.8 | |
Redundancy | 7.1 | 7.3 |
CC(1/2) | 0.999 | 0.907 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM CHES-NaOH, pH 9.5, and 30%(w/v) PEG 3000 to which 5%(v/v) of 1 M Bicine, pH8.5, was added |