7Y8R

The nucleosome-bound human PBAF complex

Summary for 7Y8R

• Entry DOI: 10.2210/pdb7y8r/pdb
• EMDB information: 33684
• Descriptor: Histone H3, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1, SWI/SNF complex subunit SMARCC2, ... (25 entities in total)
• Functional Keywords: chromatin remodeling complexes, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 27
• Total formula weight: 1458645.64

Authors

Wang, L.,Yu, J.,Yu, Z.,Wang, Q.,He, S.,Xu, Y. (deposition date: 2022-06-24, release date: 2022-12-07, Last modification date: 2024-07-03)

Primary citation

Wang, L.,Yu, J.,Yu, Z.,Wang, Q.,Li, W.,Ren, Y.,Chen, Z.,He, S.,Xu, Y.
Structure of nucleosome-bound human PBAF complex.
Nat Commun, 13:7644-7644, 2022

PubMed Abstract: BAF and PBAF are mammalian SWI/SNF family chromatin remodeling complexes that possess multiple histone/DNA-binding subunits and create nucleosome-depleted/free regions for transcription activation. Despite previous structural studies and recent advance of SWI/SNF family complexes, it remains incompletely understood how PBAF-nucleosome complex is organized. Here we determined structure of 13-subunit human PBAF in complex with acetylated nucleosome in ADP-BeF-bound state. Four PBAF-specific subunits work together with nine BAF/PBAF-shared subunits to generate PBAF-specific modular organization, distinct from that of BAF at various regions. PBAF-nucleosome structure reveals six histone-binding domains and four DNA-binding domains/modules, the majority of which directly bind histone/DNA. This multivalent nucleosome-binding pattern, not observed in previous studies, suggests that PBAF may integrate comprehensive chromatin information to target genomic loci for function. Our study reveals molecular organization of subunits and histone/DNA-binding domains/modules in PBAF-nucleosome complex and provides structural insights into PBAF-mediated nucleosome association complimentary to the recently reported PBAF-nucleosome structure.

PubMed: 36496390
DOI: 10.1038/s41467-022-34859-5

Experimental method

ELECTRON MICROSCOPY (4.4 Å)