7Y8P

Crystal structure of 4'-selenoRNA duplex

Summary for 7Y8P

• Entry DOI: 10.2210/pdb7y8p/pdb
• Descriptor: RNA (5'-R(*GP*GP*AP*(IKS)P*(ILK)P*(IKS)P*GP*AP*GP*UP*CP*C)-3'), COBALT HEXAMMINE(III) (3 entities in total)
• Functional Keywords: rna, selen
• Biological source: synthetic construct
• Total number of polymer chains: 2
• Total formula weight: 8515.78

Authors

Kondo, J.,Minakawa, N.,Ohta, M.,Takahashi, H.,Tarashima, N. (deposition date: 2022-06-24, release date: 2023-05-03, Last modification date: 2023-11-29)

Primary citation

Ota, M.,Takahashi, H.,Nogi, Y.,Kagotani, Y.,Saito-Tarashima, N.,Kondo, J.,Minakawa, N.
Synthesis and properties of fully-modified 4'-selenoRNA, an endonuclease-resistant RNA analog.
Bioorg.Med.Chem., 76:117093-117093, 2022

PubMed Abstract: A large number of chemically modified oligonucleotides (ONs) have been developed for RNA-based technologies. In most modified RNAs, the characteristic 2'-hydroxyl (2'-OH) groups are removed to enhance both nuclease resistance and hybridization ability. However, the importance of the 2'-OH group in RNA structure and function is well known. Here, we report the synthesis and properties of 4'-selenoRNA in which all four nucleoside units retain the 2'-OH groups but contain a selenium atom instead of an oxygen atom at the 4'-position of the furanose ring. 4'-SelenoRNA has enhanced ability to form duplexes with RNA, and high endonuclease resistance despite the presence of the 2'-OH groups. X-ray crystallography analysis showed that the 4'-selenoRNA duplex adopts an A-conformation, similar to natural RNA, although one 4'-selenocytidine residue has unusual South-type sugar puckering. Furthermore, preliminary studies using 4'-seleno-modified siRNAs suggest that 4'-selenoRNA may be applicable to RNA interference technology. Collectively, our results raise the possibility of a new class of modified RNA in which 2'-OH groups do not need to be masked.

PubMed: 36434923
DOI: 10.1016/j.bmc.2022.117093

Experimental method

X-RAY DIFFRACTION (1.5 Å)