7Y8P
Crystal structure of 4'-selenoRNA duplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-23 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 26.951, 38.107, 31.882 |
Unit cell angles | 90.00, 113.47, 90.00 |
Refinement procedure
Resolution | 29.250 - 1.500 |
R-factor | 0.1634 |
Rwork | 0.160 |
R-free | 0.19860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ay2 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.250 | 29.250 | 1.540 |
High resolution limit [Å] | 1.500 | 6.700 | 1.500 |
Rmerge | 0.080 | 0.053 | 0.281 |
Rmeas | 0.095 | 0.064 | 0.336 |
Total number of observations | 62087 | ||
Number of reflections | 18427 | 200 | 1312 |
<I/σ(I)> | 11.18 | 21.16 | 4.24 |
Completeness [%] | 98.5 | 94.8 | 96.8 |
Redundancy | 3.369 | 3.225 | 3.317 |
CC(1/2) | 0.994 | 0.994 | 0.946 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | MPD, sodium cacodylate, hexammine cobalt |