7XRB

human STK19 dimer

Summary for 7XRB

• Entry DOI: 10.2210/pdb7xrb/pdb
• Descriptor: Isoform 2 of Serine/threonine-protein kinase 19, SULFATE ION, CHLORIDE ION, ... (4 entities in total)
• Functional Keywords: winged helix domain, nucleus protein, dna binding protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 52481.74

Authors

Sun, Q.,Li, Y. (deposition date: 2022-05-10, release date: 2023-06-07, Last modification date: 2025-02-26)

Primary citation

Li, Y.,Gong, Y.,Zhou, Y.,Xiao, Y.,Huang, W.,Zhou, Q.,Tu, Y.,Zhao, Y.,Zhang, S.,Dai, L.,Sun, Q.
STK19 is a DNA/RNA-binding protein critical for DNA damage repair and cell proliferation.
J.Cell Biol., 223:-, 2024

PubMed Abstract: STK19 was originally identified as a manganese-dependent serine/threonine-specific protein kinase, but its function has been highly debated. Here, the crystal structure of STK19 revealed that it does not contain a kinase domain, but three intimately packed winged helix (WH) domains. The third WH domain mediated homodimerization and double-stranded DNA binding, both being important for its nuclear localization. STK19 participated in the nucleotide excision repair (NER) and mismatch repair (MMR) pathways by recruiting damage repair factors such as RPA2 and PCNA. STK19 also bound double-stranded RNA through the DNA-binding interface and regulated the expression levels of many mRNAs. Furthermore, STK19 knockdown cells exhibited very slow cell proliferation, which cannot be rescued by dimerization or DNA-binding mutants. Therefore, this work concludes that STK19 is highly unlikely to be a kinase but a DNA/RNA-binding protein critical for DNA damage repair (DDR) and cell proliferation. To prevent further confusions, we renamed this protein as TWH19 (Tandem Winged Helix protein formerly known as STK19).

PubMed: 38252411
DOI: 10.1083/jcb.202301090

Experimental method

X-RAY DIFFRACTION (1.65 Å)