7XRB
human STK19 dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-10-24 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.330, 96.620, 98.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.090 - 1.650 |
| R-factor | 0.1512 |
| Rwork | 0.149 |
| R-free | 0.19380 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.487 |
| Data reduction software | HKL-3000 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | RESOLVE |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 69.090 | 69.090 | 1.690 |
| High resolution limit [Å] | 1.650 | 7.380 | 1.650 |
| Rmerge | 0.064 | 0.053 | 0.937 |
| Rmeas | 0.067 | 0.056 | 0.991 |
| Rpim | 0.019 | 0.017 | 0.310 |
| Total number of observations | 800904 | 7799 | 45192 |
| Number of reflections | 64082 | 806 | 4673 |
| <I/σ(I)> | 26.1 | 53.7 | 2.4 |
| Completeness [%] | 99.3 | 97.5 | 99.2 |
| Redundancy | 12.5 | 9.7 | 9.7 |
| CC(1/2) | 0.999 | 0.998 | 0.821 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2 M ammonium sulfate, 0.1 M MES pH 6.5, 0.2 M NaCl |
