7XHN
Structure of human inner kinetochore CCAN-DNA complex
Summary for 7XHN
| Entry DOI | 10.2210/pdb7xhn/pdb |
| EMDB information | 33196 |
| Descriptor | Centromere protein O, Centromere protein T, CENP-W, ... (18 entities in total) |
| Functional Keywords | cell cycle |
| Biological source | Homo sapiens (human) More |
| Total number of polymer chains | 20 |
| Total formula weight | 772181.15 |
| Authors | Sun, L.F.,Tian, T.,Wang, C.L.,Yang, Z.S.,Zang, J.Y. (deposition date: 2022-04-09, release date: 2023-01-25, Last modification date: 2024-07-03) |
| Primary citation | Tian, T.,Chen, L.,Dou, Z.,Yang, Z.,Gao, X.,Yuan, X.,Wang, C.,Liu, R.,Shen, Z.,Gui, P.,Teng, M.,Meng, X.,Hill, D.L.,Li, L.,Zhang, X.,Liu, X.,Sun, L.,Zang, J.,Yao, X. Structural insights into human CCAN complex assembled onto DNA. Cell Discov, 8:90-90, 2022 Cited by PubMed Abstract: In mitosis, accurate chromosome segregation depends on kinetochores that connect centromeric chromatin to spindle microtubules. The centromeres of budding yeast, which are relatively simple, are connected to individual microtubules via a kinetochore constitutive centromere associated network (CCAN). However, the complex centromeres of human chromosomes comprise millions of DNA base pairs and attach to multiple microtubules. Here, by use of cryo-electron microscopy and functional analyses, we reveal the molecular basis of how human CCAN interacts with duplex DNA and facilitates accurate chromosome segregation. The overall structure relates to the cooperative interactions and interdependency of the constituent sub-complexes of the CCAN. The duplex DNA is topologically entrapped by human CCAN. Further, CENP-N does not bind to the RG-loop of CENP-A but to DNA in the CCAN complex. The DNA binding activity is essential for CENP-LN localization to centromere and chromosome segregation during mitosis. Thus, these analyses provide new insights into mechanisms of action underlying kinetochore assembly and function in mitosis. PubMed: 36085283DOI: 10.1038/s41421-022-00439-6 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.71 Å) |
Structure validation
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