7X5Q

Apo Truncated VhChiP (Delta 1-19) in complex with peptide (DGANSDAAK)

Summary for 7X5Q

• Entry DOI: 10.2210/pdb7x5q/pdb
• Descriptor: Chitoporin, ASP-GLY-ALA-ASN-SER-ASP, (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE, ... (5 entities in total)
• Functional Keywords: vibrio species, chitin, outer-membrane protein, sugar-specific porin, marine bacteria, transport protein
• Biological source: Vibrio harveyi; More
• Total number of polymer chains: 7
• Total formula weight: 222520.52

Authors

Sanram, S.,Aunkham, A.,Suginta, W.,Robinson, R.C. (deposition date: 2022-03-05, release date: 2023-03-08, Last modification date: 2025-01-29)

Primary citation

Sanram, S.,Aunkham, A.,Robinson, R.,Suginta, W.
Structural displacement model of chitooligosaccharide transport through chitoporin.
J.Biol.Chem., 299:105000-105000, 2023

PubMed Abstract: VhChiP is a chitooligosaccharide-specific porin identified in the outer membrane of Vibrio campbellii type strain American Type Culture Collection BAA 1116. VhChiP contains three identical subunits, and in each subunit, the 19-amino acid N-terminal segment serves as a molecular plug (the "N-plug") that controls the closed/open dynamics of the neighboring pores. In this study, the crystal structures of VhChiP lacking the N-plug were determined in the absence and presence of chitohexaose. Binding studies of sugar-ligand interactions by single-channel recordings and isothermal microcalorimetry experiments suggested that the deletion of the N-plug peptide significantly weakened the sugar-binding affinity due to the loss of hydrogen bonds around the central affinity sites. Steered molecular dynamic simulations revealed that the movement of the sugar chain along the sugar passage triggered the ejection of the N-plug, while the H-bonds transiently formed between the reducing end GlcNAc units of the sugar chain with the N-plug peptide may help to facilitate sugar translocation. The findings enable us to propose the structural displacement model, which enables us to understand the molecular basis of chitooligosaccharide uptake by marine Vibrio bacteria.

PubMed: 37394001
DOI: 10.1016/j.jbc.2023.105000

Experimental method

X-RAY DIFFRACTION (2.7 Å)