7X5Q
Apo Truncated VhChiP (Delta 1-19) in complex with peptide (DGANSDAAK)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2020-08-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.085, 182.602, 196.808 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.870 - 2.700 |
R-factor | 0.2128 |
Rwork | 0.210 |
R-free | 0.25610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mdo |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.700 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmeas | 0.331 | 0.331 |
Rpim | 0.089 | 0.819 |
Number of reflections | 84382 | 84382 |
<I/σ(I)> | 14.2 | 14.2 |
Completeness [%] | 97.3 | |
Redundancy | 7.2 | |
CC(1/2) | 0.752 | 0.504 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 292 | 0.1M Magnesium nitrate hexahydrate, 0.05M Magnesium acetate tetrahydrate, 0.1M Sodium citrate pH 5.0, and 18% w/vPEG 1500 |