7X4O
Crystal structure of Vps17p PX from S. cerevisiae (Space)
Summary for 7X4O
| Entry DOI | 10.2210/pdb7x4o/pdb |
| Descriptor | Vacuolar protein sorting-associated protein 17, SULFATE ION (3 entities in total) |
| Functional Keywords | phox domain crystallized in international space station, lipid binding protein |
| Biological source | Saccharomyces cerevisiae S288C (baker's yeast) |
| Total number of polymer chains | 2 |
| Total formula weight | 32754.90 |
| Authors | |
| Primary citation | Obita, T.,Inaka, K.,Kohda, D.,Maita, N. Crystal structure of the PX domain of Vps17p from Saccharomyces cerevisiae. Acta Crystallogr.,Sect.F, 78:210-216, 2022 Cited by PubMed Abstract: The structure determination of the PX (phox homology) domain of the Saccharomyces cerevisiae Vps17p protein presented a challenging case for molecular replacement because it has noncrystallographic symmetry close to a crystallographic axis. The combination of diffraction-quality crystals grown under microgravity on the International Space Station and a highly accurate template structure predicted by AlphaFold2 provided the key to successful crystal structure determination. Although the structure of the Vps17p PX domain is seen in many PX domains, no basic residues are found around the canonical phosphatidylinositol phosphate (PtdIns-P) binding site, suggesting an inability to bind PtdIns-P molecules. PubMed: 35506766DOI: 10.1107/S2053230X22004472 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.02 Å) |
Structure validation
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