7WB0
PlmCasX-sgRNAv1-dsDNA ternary complex at nts loading state with flexible H2 domain
Summary for 7WB0
Entry DOI | 10.2210/pdb7wb0/pdb |
EMDB information | 32391 |
Descriptor | TS-DNA, NTS-DNA, RNA (115-MER), ... (4 entities in total) |
Functional Keywords | crispr, casx, sgrna, r-loop complex, rna binding protein, dna binding protein, rna binding protein-rna-dna complex, rna binding protein/rna/dna |
Biological source | Planctomycetes bacterium More |
Total number of polymer chains | 4 |
Total formula weight | 176432.68 |
Authors | Zhang, S.,Liu, J.J.G. (deposition date: 2021-12-15, release date: 2022-03-16, Last modification date: 2022-03-30) |
Primary citation | Tsuchida, C.A.,Zhang, S.,Doost, M.S.,Zhao, Y.,Wang, J.,O'Brien, E.,Fang, H.,Li, C.P.,Li, D.,Hai, Z.Y.,Chuck, J.,Brotzmann, J.,Vartoumian, A.,Burstein, D.,Chen, X.W.,Nogales, E.,Doudna, J.A.,Liu, J.G. Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity. Mol.Cell, 82:1199-1209.e6, 2022 Cited by PubMed: 35219382DOI: 10.1016/j.molcel.2022.02.002 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (3.2 Å) |
Structure validation
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