7WB0
PlmCasX-sgRNAv1-dsDNA ternary complex at nts loading state with flexible H2 domain
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.2 |
Software | cryoSPARC |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |