7WAG
Crystal structure of MurJ squeezed form
Summary for 7WAG
| Entry DOI | 10.2210/pdb7wag/pdb |
| Descriptor | Lipid II flippase MurJ, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate, DI(HYDROXYETHYL)ETHER, ... (4 entities in total) |
| Functional Keywords | 14 transmembrane helices, inner membrane, lipid ii, mop superfamily, lipid transport |
| Biological source | Escherichia coli K-12 |
| Total number of polymer chains | 1 |
| Total formula weight | 61320.55 |
| Authors | Tsukazaki, T.,Kohga, H.,Tanaka, Y.,Yoshikaie, K.,Taniguchi, K.,Fujimoto, K. (deposition date: 2021-12-14, release date: 2022-06-01, Last modification date: 2023-11-29) |
| Primary citation | Kohga, H.,Mori, T.,Tanaka, Y.,Yoshikaie, K.,Taniguchi, K.,Fujimoto, K.,Fritz, L.,Schneider, T.,Tsukazaki, T. Crystal structure of the lipid flippase MurJ in a "squeezed" form distinct from its inward- and outward-facing forms. Structure, 30:1088-1097.e3, 2022 Cited by PubMed Abstract: The bacterial peptidoglycan enclosing the cytoplasmic membrane is a fundamental cellular architecture. The integral membrane protein MurJ plays an essential role in flipping the cell wall building block Lipid II across the cytoplasmic membrane for peptidoglycan biosynthesis. Previously reported crystal structures of MurJ have elucidated its V-shaped inward- or outward-facing forms with an internal cavity for substrate binding. MurJ transports Lipid II using its cavity through conformational transitions between these two forms. Here, we report two crystal structures of inward-facing forms from Arsenophonus endosymbiont MurJ and an unprecedented crystal structure of Escherichia coli MurJ in a "squeezed" form, which lacks a cavity to accommodate the substrate, mainly because of the increased proximity of transmembrane helices 2 and 8. Subsequent molecular dynamics simulations supported the hypothesis that the squeezed form is an intermediate conformation. This study fills a gap in our understanding of the Lipid II flipping mechanism. PubMed: 35660157DOI: 10.1016/j.str.2022.05.008 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.55 Å) |
Structure validation
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