7WAG
Crystal structure of MurJ squeezed form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-26 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.450, 75.060, 115.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.700 - 2.550 |
R-factor | 0.2195 |
Rwork | 0.214 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6cc4 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.778 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.700 | 2.640 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 21690 | 2154 |
<I/σ(I)> | 10.98 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 35.7 | |
CC(1/2) | 0.996 | 0.502 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293.15 | MPD, MgCl2, Tris-HCl, PEG, OLC |