7W5Z

Cryo-EM structure of Tetrahymena thermophila mitochondrial complex IV, composite dimer model

This is a non-PDB format compatible entry.

Summary for 7W5Z

• Entry DOI: 10.2210/pdb7w5z/pdb
• EMDB information: 32325
• Descriptor: Unknown peptide, Cytochrome C oxidase subunit Vb protein, Transmembrane protein, putative, ... (65 entities in total)
• Functional Keywords: cytochrome c oxidase, electron transport, mitochondrial carrier, membrane complex
• Biological source: Tetrahymena thermophila; More
• Total number of polymer chains: 116
• Total formula weight: 4593537.45

Authors

Zhou, L.,Maldonado, M.,Padavannil, A.,Letts, J. (deposition date: 2021-11-30, release date: 2022-04-06, Last modification date: 2024-10-23)

Primary citation

Zhou, L.,Maldonado, M.,Padavannil, A.,Guo, F.,Letts, J.A.
Structures of Tetrahymena 's respiratory chain reveal the diversity of eukaryotic core metabolism.
Science, 376:831-839, 2022

PubMed Abstract: Respiration is a core biological energy-converting process whose last steps are carried out by a chain of multisubunit complexes in the inner mitochondrial membrane. To probe the functional and structural diversity of eukaryotic respiration, we examined the respiratory chain of the ciliate (Tt). Using cryo-electron microscopy on a mixed sample, we solved structures of a supercomplex between Tt complex I (Tt-CI) and Tt-CIII (Tt-SC I+III) and a structure of Tt-CIV. Tt-SC I+III (~2.3 megadaltons) is a curved assembly with structural and functional symmetry breaking. Tt-CIV is a ~2.7-megadalton dimer with more than 50 subunits per protomer, including mitochondrial carriers and a TIM8-TIM13-like domain. Our structural and functional study of the respiratory chain reveals divergence in key components of eukaryotic respiration, thereby expanding our understanding of core metabolism.

PubMed: 35357889
DOI: 10.1126/science.abn7747

Experimental method

ELECTRON MICROSCOPY (3.02 Å)