7W5Z

Cryo-EM structure of Tetrahymena thermophila mitochondrial complex IV, composite dimer model

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	U1, u1	Unknown peptide	polymer	98	8358.3	2		Tetrahymena thermophila
2	U2, u2	Transmembrane protein, putative	polymer	3634	417001.6	2	UniProt (Q22C06) In PDB	Tetrahymena thermophila
3	U3, u3	Unknown peptide	polymer	34	2911.6	2		Tetrahymena thermophila
4	U4, u4	Unknown peptide	polymer	30	2571.2	2		Tetrahymena thermophila
5	U5, u5	Uncharacterized protein	polymer	172	20909.6	2	UniProt (Q23FE4) Pfam (PF14536) In PDB	Tetrahymena thermophila
6	U6, u6	Protein transporter Sec61 alpha subunit	polymer	478	53674.3	2	UniProt (W7XHC8) Pfam (PF10559) Pfam (PF00344) In PDB	Tetrahymena thermophila
7	C1, c1	Cytochrome c oxidase subunit 1	polymer	688	80564.9	2	UniProt (Q950Y4) Pfam (PF00115) In PDB	Tetrahymena thermophila
8	C2, c2	Cytochrome c oxidase subunit 2	polymer	604	72388.9	2	UniProt (Q950Y9) Pfam (PF00116) In PDB	Tetrahymena thermophila
9	C3, c3	Ymf68	polymer	594	72782.5	2	UniProt (Q950Y6) In PDB	Tetrahymena thermophila
10	5B, 5b	Cytochrome C oxidase subunit Vb protein	polymer	637	75431.3	2	UniProt (Q23FF5) Pfam (PF01215) In PDB	Tetrahymena thermophila
11	6A, 6a	Transmembrane protein, putative	polymer	130	15618.3	2	UniProt (W7XCY5) In PDB	Tetrahymena thermophila
12	6B, 6b	Cytochrome c oxidase subunit 6B	polymer	230	27946.4	2	UniProt (Q24I72) In PDB	Tetrahymena thermophila
13	6L, 6l	Cytochrome c oxidase subunit 6B-like	polymer	88	10228.7	2	UniProt (I7LVX0) In PDB	Tetrahymena thermophila
14	6C, 6c	Transmembrane protein, putative	polymer	103	12786.3	2	UniProt (Q23DS4) In PDB	Tetrahymena thermophila
15	7A, 7a	Transmembrane protein, putative	polymer	133	16413.6	2	UniProt (I7MGF9) In PDB	Tetrahymena thermophila
16	7C, 7c	Cytochrome c oxidase subunit 7C	polymer	236	28746.6	2	UniProt (W7X287) In PDB	Tetrahymena thermophila
17	7L, 7l	CTF/NF-I domain-containing protein	polymer	990	116812.8	2	UniProt (Q22CI1) In PDB	Tetrahymena thermophila
18	M1, m1	Oxoglutarate/malate translocator protein, putative	polymer	346	40392.2	2	UniProt (A4VDV3) Pfam (PF00153) In PDB	Tetrahymena thermophila
19	M2, m2	2-oxoglutarate/malate carrier protein	polymer	318	36118.3	2	UniProt (Q23M99) Pfam (PF00153) In PDB	Tetrahymena thermophila
20	M3, m3	Carrier protein	polymer	330	37095.1	2	UniProt (Q22ZA6) Pfam (PF00153) In PDB	Tetrahymena thermophila
21	T1, t1	Tim10/DDP family zinc finger protein	polymer	72	8009.0	2	UniProt (W7X3D6) Pfam (PF02953) In PDB	Tetrahymena thermophila
22	T2, t2	Cytochrome c oxidase small TIM subunit 2	polymer	72	8354.6	2	UniProt (W7XDM6) In PDB	Tetrahymena thermophila
23	T3, t3	Cytochrome c oxidase small TIM subunit 3	polymer	93	10435.7	2	UniProt (Q231A8) In PDB	Tetrahymena thermophila
24	T4, t4	Cytochrome c oxidase small TIM subunit 4	polymer	68	8109.1	2	UniProt (Q22A35) In PDB	Tetrahymena thermophila
25	T5, t5	Cytochrome c oxidase small TIM subunit 5	polymer	81	8965.9	2	UniProt (Q22N23) In PDB	Tetrahymena thermophila
26	T6, t6	Cytochrome c oxidase small TIM subunit 6	polymer	72	8183.4	2	UniProt (Q233U0) In PDB	Tetrahymena thermophila
27	BP, bp	Chromosome condensation regulator RCC1 repeat protein	polymer	380	41202.6	2	UniProt (Q22RF2) Pfam (PF00415) In PDB	Tetrahymena thermophila
28	FS, fs	Iron-binding zinc finger CDGSH type protein	polymer	188	21476.9	2	UniProt (I7M8P0) In PDB	Tetrahymena thermophila
29	AC, ac	Cytochrome c oxidase acyl carrier-like subunit	polymer	127	14740.9	2	UniProt (Q24C97) In PDB	Tetrahymena thermophila
30	Y7, y7	Ymf67	polymer	453	54538.5	2	UniProt (Q950Y7) In PDB	Tetrahymena thermophila
31	Y0, y0	Ymf70	polymer	89	10919.9	2	UniProt (Q950Y0) In PDB	Tetrahymena thermophila
32	Y5, y5	Ymf75	polymer	190	23325.8	2	UniProt (Q951A7) In PDB	Tetrahymena thermophila
33	A, a	Transmembrane protein, putative	polymer	490	57814.5	2	UniProt (Q22PJ5) In PDB	Tetrahymena thermophila
34	B, b	Protein phosphatase 2C, putative	polymer	473	53011.3	2	UniProt (Q22FX8) In PDB	Tetrahymena thermophila
35	C, c	Cyclic nucleotide-binding domain protein	polymer	1471	172272.8	2	UniProt (A4VCQ2) Pfam (PF00520) Pfam (PF07885) Pfam (PF00027) Pfam (PF00098) In PDB	Tetrahymena thermophila
36	D, d	SURF1-like protein	polymer	402	47088.0	2	UniProt (I7M1Q4) Pfam (PF02104) In PDB	Tetrahymena thermophila
37	E, e	TraB family protein	polymer	385	45050.9	2	UniProt (Q23DP7) In PDB	Tetrahymena thermophila
38	F, f	Transmembrane protein, putative	polymer	348	41307.4	2	UniProt (Q23DG8) In PDB	Tetrahymena thermophila
39	G, g	Cytochrome c oxidase subunit TT7	polymer	318	37553.0	2	UniProt (Q23DZ5) In PDB	Tetrahymena thermophila
40	H, h	SURF1-like protein	polymer	318	35794.4	2	UniProt (I7LTZ4) Pfam (PF02104) In PDB	Tetrahymena thermophila
41	I, i	Cytochrome c oxidase subunit TT9	polymer	252	29865.7	2	UniProt (I7LY65) In PDB	Tetrahymena thermophila
42	J, j	Cytochrome c oxidase subunit TT10	polymer	234	28101.5	2	UniProt (I7MD70) In PDB	Tetrahymena thermophila
43	K, k	Cytochrome c oxidase subunit TT11	polymer	231	27034.7	2	UniProt (W7X4J9) In PDB	Tetrahymena thermophila
44	L, l	Cytochrome c oxidase subunit TT12	polymer	222	26738.4	2	UniProt (I7M3P9) In PDB	Tetrahymena thermophila
45	M, m	Transmembrane protein, putative	polymer	220	26601.2	2	UniProt (W7WZP1) In PDB	Tetrahymena thermophila
46	N, n	Transmembrane protein, putative	polymer	210	24701.1	2	UniProt (I7LZX8) In PDB	Tetrahymena thermophila
47	O, o	Cytochrome c oxidase subunit TT15	polymer	193	23257.7	2	UniProt (Q23F08) In PDB	Tetrahymena thermophila
48	P, p	Cytochrome c oxidase subunit TT16	polymer	175	20007.3	2	UniProt (I7M8Y9) In PDB	Tetrahymena thermophila
49	Q, q	Transmembrane protein, putative	polymer	173	20346.2	2	UniProt (Q23D87) In PDB	Tetrahymena thermophila
50	R, r	Cytochrome c oxidase subunit TT18	polymer	173	20025.1	2	UniProt (I7MKT6) In PDB	Tetrahymena thermophila
51	S, s	Cytochrome c oxidase subunit TT19	polymer	170	19882.8	2	UniProt (Q230X6) In PDB	Tetrahymena thermophila
52	T, t	Transmembrane protein, putative	polymer	158	18801.6	2	UniProt (Q23VY4) In PDB	Tetrahymena thermophila
53	U, u	Transmembrane protein, putative	polymer	154	18600.4	2	UniProt (Q22DP8) In PDB	Tetrahymena thermophila
54	V, v	Cytochrome c oxidase subunit TT22	polymer	149	17701.1	2	UniProt (I7MFV5) In PDB	Tetrahymena thermophila
55	W, w	Transmembrane protein, putative	polymer	124	15588.6	2	UniProt (Q23TE5) In PDB	Tetrahymena thermophila
56	X, x	Transmembrane protein, putative	polymer	122	14302.5	2	UniProt (Q22W32) In PDB	Tetrahymena thermophila
57	Y, y	Cytochrome c oxidase subunit TT25	polymer	105	12273.9	2	UniProt (I7M9E7) In PDB	Tetrahymena thermophila
58	Z, z	Cytochrome c oxidase subunit TT26	polymer	90	10418.7	2	UniProt (I7LTF1) In PDB	Tetrahymena thermophila
59	C1, c1	HEME-A	non-polymer		852.8	4	Chemie (HEA)
60	c2, C1, C2, c1	COPPER (II) ION	non-polymer		63.5	6	Chemie (CU)
61	C1, c1	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
62	m2, A, v, c3, 7C...	1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE	non-polymer		790.1	34	Chemie (PC1)
63	Y0, c1, t, 5B, Y5...	CARDIOLIPIN	non-polymer		1464.0	68	Chemie (CDL)
64	5B, 5b, M, m	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
65	fs, FS	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	4	Chemie (FES)

Sequence modifications

BP, bp: 83 - 412 (UniProt: Q22RF2)

PDB	External Database	Details
Glu 413	-	expression tag
Thr 414	-	expression tag
Gly 415	-	expression tag
Lys 416	-	expression tag
Ile 417	-	expression tag
Tyr 418	-	expression tag
Gln 419	-	expression tag
Phe 420	-	expression tag
Asn 421	-	expression tag
Glu 422	-	expression tag
Phe 423	-	expression tag
Val 424	-	expression tag
Gly 425	-	expression tag
Val 426	-	expression tag
Ser 427	-	expression tag
Thr 428	-	expression tag
Asn 429	-	expression tag
Glu 430	-	expression tag
Val 431	-	expression tag
Gly 432	-	expression tag
Asn 433	-	expression tag
Asp 434	-	expression tag
Tyr 435	-	expression tag
Asn 436	-	expression tag
Val 437	-	expression tag
Ala 438	-	expression tag
Asp 439	-	expression tag
Ser 440	-	expression tag
Lys 441	-	expression tag
Ala 442	-	expression tag
Phe 443	-	expression tag
Glu 444	-	expression tag
Gly 445	-	expression tag
Lys 446	-	expression tag
Val 447	-	expression tag
Val 448	-	expression tag
Asp 449	-	expression tag
Leu 450	-	expression tag
Gly 451	-	expression tag
Gly 452	-	expression tag
Ser 453	-	expression tag
Tyr 454	-	expression tag
Gly 455	-	expression tag
Ile 456	-	expression tag
Arg 457	-	expression tag
Phe 458	-	expression tag
Ala 459	-	expression tag
Ile 460	-	expression tag
Val 461	-	expression tag
Asn 462	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	116
	Total formula weight	4462311.4
Non-Polymers*	Number of molecules	122
	Total formula weight	131226.0
All*	Total formula weight	4593537.4

*Water molecules are not included.