7W4O

The structure of KATP H175K mutant in pre-open state

Summary for 7W4O

• Entry DOI: 10.2210/pdb7w4o/pdb
• EMDB information: 32310
• Descriptor: ATP-sensitive inward rectifier potassium channel 11, ATP-binding cassette sub-family C member 8 isoform X2, ADENOSINE-5'-DIPHOSPHATE, ... (6 entities in total)
• Functional Keywords: katp, kir6.2, sur1, nn414, adp, atp, transport protein
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 8
• Total formula weight: 888708.22

Authors

Chen, L.,Wang, M. (deposition date: 2021-11-28, release date: 2022-06-01, Last modification date: 2024-10-16)

Primary citation

Wang, M.,Wu, J.X.,Ding, D.,Chen, L.
Structural insights into the mechanism of pancreatic K ATP channel regulation by nucleotides.
Nat Commun, 13:2770-2770, 2022

PubMed Abstract: ATP-sensitive potassium channels (K) are metabolic sensors that convert the intracellular ATP/ADP ratio to the excitability of cells. They are involved in many physiological processes and implicated in several human diseases. Here we present the cryo-EM structures of the pancreatic K channel in both the closed state and the pre-open state, resolved in the same sample. We observe the binding of nucleotides at the inhibitory sites of the Kir6.2 channel in the closed but not in the pre-open state. Structural comparisons reveal the mechanism for ATP inhibition and Mg-ADP activation, two fundamental properties of K channels. Moreover, the structures also uncover the activation mechanism of diazoxide-type K openers.

PubMed: 35589716
DOI: 10.1038/s41467-022-30430-4

Experimental method

ELECTRON MICROSCOPY (2.96 Å)