7V9I

The Monomer mutant of BEN4 domain of protein Bend3 with DNA

Summary for 7V9I

• Entry DOI: 10.2210/pdb7v9i/pdb
• Descriptor: BEN domain-containing protein 3, DNA (5'-D(*GP*CP*CP*CP*CP*AP*CP*GP*CP*GP*GP*T)-3'), DNA (5'-D(*AP*CP*CP*GP*CP*GP*TP*GP*GP*GP*GP*C)-3') (3 entities in total)
• Functional Keywords: dna binding protein-dna complex, dna binding protein/dna
• Biological source: Mus musculus (Mouse); More
• Total number of polymer chains: 3
• Total formula weight: 20862.54

Authors

Zhang, J.,Zhang, Y.,You, Q.,Huang, C.,Zhang, T.,Wang, M.,Zhang, T.,Yang, X.,Xiong, J.,Li, Y.,Liu, C.P.,Zhang, Z.,Xu, R.M.,Zhu, B. (deposition date: 2021-08-25, release date: 2022-02-16, Last modification date: 2024-05-29)

Primary citation

Zhang, J.,Zhang, Y.,You, Q.,Huang, C.,Zhang, T.,Wang, M.,Zhang, T.,Yang, X.,Xiong, J.,Li, Y.,Liu, C.P.,Zhang, Z.,Xu, R.M.,Zhu, B.
Highly enriched BEND3 prevents the premature activation of bivalent genes during differentiation.
Science, 375:1053-1058, 2022

PubMed Abstract: Bivalent genes are ready for activation upon the arrival of developmental cues. Here, we report that BEND3 is a CpG island (CGI)-binding protein that is enriched at regulatory elements. The cocrystal structure of BEND3 in complex with its target DNA reveals the structural basis for its DNA methylation-sensitive binding property. Mouse embryos ablated of died at the pregastrulation stage. null embryonic stem cells (ESCs) exhibited severe defects in differentiation, during which hundreds of CGI-containing bivalent genes were prematurely activated. BEND3 is required for the stable association of polycomb repressive complex 2 (PRC2) at bivalent genes that are highly occupied by BEND3, which suggests a reining function of BEND3 in maintaining high levels of H3K27me3 at these bivalent genes in ESCs to prevent their premature activation in the forthcoming developmental stage.

PubMed: 35143257
DOI: 10.1126/science.abm0730

Experimental method

X-RAY DIFFRACTION (3.5 Å)