7V9I

The Monomer mutant of BEN4 domain of protein Bend3 with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-10
Detector	MAR CCD 165 mm
Wavelength(s)	1.5420
Spacegroup name	C 2 2 21
Unit cell lengths	51.504, 61.990, 136.024
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.010 - 3.500
R-factor	0.2139
Rwork	0.211
R-free	0.26080
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.630
High resolution limit [Å]	3.500	3.500
Rmerge	0.209	0.461
Number of reflections	2848	292
<I/σ(I)>	6.1
Completeness [%]	95.6
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M Sodium formate, 20% w/v PEG 3350