7USD

Cryo-EM structure of D-site Rac1-bound WAVE Regulatory Complex

7USD の概要

• エントリーDOI: 10.2210/pdb7usd/pdb
• EMDBエントリー: 26733
• 分子名称: Cytoplasmic FMR1-interacting protein 1, Nck-associated protein 1, Wiskott-Aldrich syndrome protein family member 1, ... (8 entities in total)
• 機能のキーワード: actin regulator, gtpase binding protein, cytoskeletal regulator, cell invasion
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 359670.25

構造登録者

Ding, B.,Yang, S.,Chen, B.,Chowdhury, S. (登録日: 2022-04-25, 公開日: 2022-09-21, 最終更新日: 2024-06-12)

主引用文献

Ding, B.,Yang, S.,Schaks, M.,Liu, Y.,Brown, A.J.,Rottner, K.,Chowdhury, S.,Chen, B.
Structures reveal a key mechanism of WAVE regulatory complex activation by Rac1 GTPase.
Nat Commun, 13:5444-5444, 2022

PubMed Abstract: The Rho-family GTPase Rac1 activates the WAVE regulatory complex (WRC) to drive Arp2/3 complex-mediated actin polymerization in many essential processes. Rac1 binds to WRC at two distinct sites-the A and D sites. Precisely how Rac1 binds and how the binding triggers WRC activation remain unknown. Here we report WRC structures by itself, and when bound to single or double Rac1 molecules, at ~3 Å resolutions by cryogenic-electron microscopy. The structures reveal that Rac1 binds to the two sites by distinct mechanisms, and binding to the A site, but not the D site, drives WRC activation. Activation involves a series of unique conformational changes leading to the release of sequestered WCA (WH2-central-acidic) polypeptide, which stimulates the Arp2/3 complex to polymerize actin. Together with biochemical and cellular analyses, the structures provide a novel mechanistic understanding of how the Rac1-WRC-Arp2/3-actin signaling axis is regulated in diverse biological processes and diseases.

PubMed: 36114192
DOI: 10.1038/s41467-022-33174-3

実験手法

ELECTRON MICROSCOPY (3 Å)