7USA
Structure of the human coronavirus CCoV-HuPn-2018 spike glycoprotein with domain 0 in the swung out conformation
This is a non-PDB format compatible entry.
Summary for 7USA
| Entry DOI | 10.2210/pdb7usa/pdb |
| EMDB information | 26730 |
| Descriptor | Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total) |
| Functional Keywords | human coronavirus, coronavirus, ccov-hupn-2018, spike glycoprotein, alpha-coronaviruses, structural genomics, seattle structural genomics center for infectious disease, ssgcid, viral protein |
| Biological source | unidentified human coronavirus |
| Total number of polymer chains | 3 |
| Total formula weight | 503931.29 |
| Authors | Tortorici, M.A.,Veesler, D.,Seattle Structural Genomics Center for Infectious Disease (SSGCID) (deposition date: 2022-04-23, release date: 2022-08-24, Last modification date: 2024-11-20) |
| Primary citation | Tortorici, M.A.,Walls, A.C.,Joshi, A.,Park, Y.J.,Eguia, R.T.,Miranda, M.C.,Kepl, E.,Dosey, A.,Stevens-Ayers, T.,Boeckh, M.J.,Telenti, A.,Lanzavecchia, A.,King, N.P.,Corti, D.,Bloom, J.D.,Veesler, D. Structure, receptor recognition, and antigenicity of the human coronavirus CCoV-HuPn-2018 spike glycoprotein. Cell, 185:2279-2291.e17, 2022 Cited by PubMed Abstract: The isolation of CCoV-HuPn-2018 from a child respiratory swab indicates that more coronaviruses are spilling over to humans than previously appreciated. We determined the structures of the CCoV-HuPn-2018 spike glycoprotein trimer in two distinct conformational states and showed that its domain 0 recognizes sialosides. We identified that the CCoV-HuPn-2018 spike binds canine, feline, and porcine aminopeptidase N (APN) orthologs, which serve as entry receptors, and determined the structure of the receptor-binding B domain in complex with canine APN. The introduction of an oligosaccharide at position N739 of human APN renders cells susceptible to CCoV-HuPn-2018 spike-mediated entry, suggesting that single-nucleotide polymorphisms might account for viral detection in some individuals. Human polyclonal plasma antibodies elicited by HCoV-229E infection and a porcine coronavirus monoclonal antibody inhibit CCoV-HuPn-2018 spike-mediated entry, underscoring the cross-neutralizing activity among ɑ-coronaviruses. These data pave the way for vaccine and therapeutic development targeting this zoonotic pathogen representing the eighth human-infecting coronavirus. PubMed: 35700730DOI: 10.1016/j.cell.2022.05.019 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.8 Å) |
Structure validation
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