7UNC
Pol II-DSIF-SPT6-PAF1c-TFIIS complex with rewrapped nucleosome
This is a non-PDB format compatible entry.
Summary for 7UNC
| Entry DOI | 10.2210/pdb7unc/pdb |
| EMDB information | 26620 |
| Descriptor | DNA-directed RNA polymerase subunit, RPB10, RPB11, ... (30 entities in total) |
| Functional Keywords | chromatin, nucleosome, transcription, intermediate |
| Biological source | Homo sapiens (human) More |
| Total number of polymer chains | 32 |
| Total formula weight | 1577916.38 |
| Authors | Filipovski, M.,Vos, S.M.,Farnung, L. (deposition date: 2022-04-10, release date: 2022-10-19, Last modification date: 2024-10-23) |
| Primary citation | Filipovski, M.,Soffers, J.H.M.,Vos, S.M.,Farnung, L. Structural basis of nucleosome retention during transcription elongation. Science, 376:1313-1316, 2022 Cited by PubMed Abstract: In eukaryotes, RNA polymerase (Pol) II transcribes chromatin and must move past nucleosomes, often resulting in nucleosome displacement. How Pol II unwraps the DNA from nucleosomes to allow transcription and how DNA rewraps to retain nucleosomes has been unclear. Here, we report the 3.0-angstrom cryo-electron microscopy structure of a mammalian Pol II-DSIF-SPT6-PAF1c-TFIIS-nucleosome complex stalled 54 base pairs within the nucleosome. The structure provides a mechanistic basis for nucleosome retention during transcription elongation where upstream DNA emerging from the Pol II cleft has rewrapped the proximal side of the nucleosome. The structure uncovers a direct role for Pol II and transcription elongation factors in nucleosome retention and explains how nucleosomes are retained to prevent the disruption of chromatin structure across actively transcribed genes. PubMed: 35709268DOI: 10.1126/science.abo3851 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3 Å) |
Structure validation
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