7UNC

Pol II-DSIF-SPT6-PAF1c-TFIIS complex with rewrapped nucleosome

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA-directed RNA polymerase subunit	polymer	1984	219049.5	1	UniProt (A0A7M4DUC2) Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990)	Sus scrofa (pig)
2	B (B)	DNA-directed RNA polymerase subunit beta	polymer	1251	142426.1	1	UniProt (A0A4X1TVZ5) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560)	Sus scrofa (pig)
3	C (C)	DNA-directed RNA polymerase II subunit RPB3	polymer	275	31439.1	1	UniProt (A0A481DF93) Pfam (PF01193) Pfam (PF01000)	Sus scrofa (pig)
4	D (D)	RPOL4c domain-containing protein	polymer	184	20962.6	1	UniProt (A0A8D1GCS3) Pfam (PF03874)	Sus scrofa (pig)
5	E (E)	DNA-directed RNA polymerase II subunit E	polymer	210	24644.3	1	UniProt (A0A4X1VTX4) Pfam (PF03871) Pfam (PF01191)	Sus scrofa (pig)	RPB5 homolog
6	F (F)	DNA-directed RNA polymerases I, II, and III subunit RPABC2	polymer	127	14477.0	1	UniProt (A0A4X1VEK9) Pfam (PF01192)	Sus scrofa (pig)	DNA-directed RNA polymerase II subunit F,RPB6 homolog
7	G (G)	DNA-directed RNA polymerase II subunit RPB7	polymer	172	19314.3	1	UniProt (A0A4X1VKG7) Pfam (PF03876) Pfam (PF00575)	Sus scrofa (pig)
8	H (H)	RPB8	polymer	150	17162.3	1		Sus scrofa
9	I (I)	RPB9	polymer	125	14541.2	1		Sus scrofa
10	J (J)	RPB10	polymer	67	7655.1	1		Sus scrofa
11	K (K)	RPB11	polymer	117	13310.3	1		Sus scrofa
12	L (L)	RNA polymerase II subunit K	polymer	58	7018.2	1	UniProt (A0A4X1TRS6) Pfam (PF03604)	Sus scrofa (pig)
13	M (M)	Transcription elongation factor SPT6	polymer	1729	199603.0	1	UniProt (Q7KZ85) Pfam (PF14632) Pfam (PF14641) Pfam (PF22706) Pfam (PF14639) Pfam (PF14635) Pfam (PF17674) Pfam (PF00575) Pfam (PF14633)	Homo sapiens (human)	hSPT6,Histone chaperone suppressor of Ty6,Tat-cotransactivator 2 protein,Tat-CT2 protein
14	N (N)	Non-template DNA	polymer	209	64763.0	1		synthetic construct
15	O (O)	Transcription elongation factor A protein 1	polymer	304	34295.0	1	UniProt (P23193) Pfam (PF08711) Pfam (PF07500) Pfam (PF01096)	Homo sapiens (human)	Transcription elongation factor S-II protein 1,Transcription elongation factor TFIIS.o
16	P (P)	RNA	polymer	16	5004.9	1		synthetic construct
17	Q (Q)	RNA polymerase-associated protein CTR9 homolog	polymer	1179	134510.2	1	UniProt (Q6PD62) Pfam (PF13374) Pfam (PF13181)	Homo sapiens (human)	SH2 domain-binding protein 1
18	R (R)	RNA polymerase-associated protein RTF1 homolog	polymer	713	80733.0	1	UniProt (Q92541) Pfam (PF03126)	Homo sapiens (human)
19	S (T)	Template DNA	polymer	215	66186.4	1		synthetic construct
20	T (U)	RNA polymerase-associated protein LEO1	polymer	666	75514.2	1	UniProt (Q8WVC0) Pfam (PF04004)	Homo sapiens (human)	Replicative senescence down-regulated leo1-like protein
21	U (V)	RNA polymerase II-associated factor 1 homolog	polymer	531	60052.7	1	UniProt (Q8N7H5) Pfam (PF03985)	Homo sapiens (human)	hPAF1,Pancreatic differentiation protein 2
22	V (W)	WDR61	polymer	305	33617.5	1	UniProt (Q9GZS3) Pfam (PF00400)	Homo sapiens (human)
23	W (X)	Parafibromin	polymer	531	60673.5	1	UniProt (Q6P1J9) Pfam (PF16050) Pfam (PF05179)	Homo sapiens (human)	Cell division cycle protein 73 homolog,Hyperparathyroidism 2 protein
24	X (Z)	Transcription elongation factor SPT5	polymer	1087	121225.5	1	UniProt (O00267) Pfam (PF11942) Pfam (PF03439) Pfam (PF00467)	Homo sapiens (human)	hSPT5,DRB sensitivity-inducing factor 160 kDa subunit,DSIF p160,DRB sensitivity-inducing factor large subunit,DSIF large subunit,Tat-cotransactivator 1 protein,Tat-CT1 protein
25	CA, Y (a, e)	Histone H3.2	polymer	136	15435.1	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)	Histone H3
26	DA, Z (b, f)	Histone H4	polymer	103	11394.4	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
27	AA, EA (c, g)	Histone H2A	polymer	130	14109.4	2	UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
28	BA, FA (d, h)	Histone H2B 1.1	polymer	123	13655.9	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
29	GA, HA, JA, KA, LA... (A, B, C, I, J...)	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
30	IA (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

M: 1 - 1723 (UniProt: Q7KZ85)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

O: 1 - 301 (UniProt: P23193)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

Q: 1 - 1173 (UniProt: Q6PD62)

PDB	External Database	Details
Glu 1174	-	expression tag
Asn 1175	-	expression tag
Leu 1176	-	expression tag
Tyr 1177	-	expression tag
Phe 1178	-	expression tag
Gln 1179	-	expression tag

R: 1 - 710 (UniProt: Q92541)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

a, e: 0 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	engineered mutation

d, h: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Met 0	-	initiating methionine
Thr 29	Ser 33	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	32
	Total formula weight	1577368.8
Non-Polymers*	Number of molecules	9
	Total formula weight	547.6
All*	Total formula weight	1577916.4

*Water molecules are not included.