7UAP
Structure of the SARS-CoV-2 S 6P trimer in complex with the neutralizing antibody Fab fragment, C1520
Summary for 7UAP
| Entry DOI | 10.2210/pdb7uap/pdb |
| EMDB information | 26429 |
| Descriptor | Spike glycoprotein, C1520 Fab Heavy Chain, C1520 Fab Light Chain, ... (5 entities in total) |
| Functional Keywords | sars-cov-2, n-terminal domain, ntd, neutralizing antibody, viral protein |
| Biological source | Severe acute respiratory syndrome coronavirus 2 More |
| Total number of polymer chains | 9 |
| Total formula weight | 575740.04 |
| Authors | Barnes, C.O. (deposition date: 2022-03-13, release date: 2022-04-27, Last modification date: 2024-11-13) |
| Primary citation | Wang, Z.,Muecksch, F.,Cho, A.,Gaebler, C.,Hoffmann, H.H.,Ramos, V.,Zong, S.,Cipolla, M.,Johnson, B.,Schmidt, F.,DaSilva, J.,Bednarski, E.,Ben Tanfous, T.,Raspe, R.,Yao, K.,Lee, Y.E.,Chen, T.,Turroja, M.,Milard, K.G.,Dizon, J.,Kaczynska, A.,Gazumyan, A.,Oliveira, T.Y.,Rice, C.M.,Caskey, M.,Bieniasz, P.D.,Hatziioannou, T.,Barnes, C.O.,Nussenzweig, M.C. Analysis of memory B cells identifies conserved neutralizing epitopes on the N-terminal domain of variant SARS-Cov-2 spike proteins. Immunity, 55:998-1012.e8, 2022 Cited by PubMed Abstract: SARS-CoV-2 infection or vaccination produces neutralizing antibody responses that contribute to better clinical outcomes. The receptor-binding domain (RBD) and the N-terminal domain (NTD) of the spike trimer (S) constitute the two major neutralizing targets for antibodies. Here, we use NTD-specific probes to capture anti-NTD memory B cells in a longitudinal cohort of infected individuals, some of whom were vaccinated. We found 6 complementation groups of neutralizing antibodies. 58% targeted epitopes outside the NTD supersite, 58% neutralized either Gamma or Omicron, and 14% were broad neutralizers that also neutralized Omicron. Structural characterization revealed that broadly active antibodies targeted three epitopes outside the NTD supersite including a class that recognized both the NTD and SD2 domain. Rapid recruitment of memory B cells producing these antibodies into the plasma cell compartment upon re-infection likely contributes to the relatively benign course of subsequent infections with SARS-CoV-2 variants, including Omicron. PubMed: 35447092DOI: 10.1016/j.immuni.2022.04.003 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.8 Å) |
Structure validation
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