7U20

Crystal structure of human METTL1 and WDR4 complex

Summary for 7U20

• Entry DOI: 10.2210/pdb7u20/pdb
• Descriptor: tRNA (guanine-N(7)-)-methyltransferase, tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4, SULFATE ION, ... (4 entities in total)
• Functional Keywords: mettl1 wdr4 trna methyltransferase, transferase
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 79167.72

Authors

Li, J.,Nowak, R.P.,Fischer, E.S.,Gregory, R. (deposition date: 2022-02-22, release date: 2022-12-07, Last modification date: 2023-10-25)

Primary citation

Li, J.,Wang, L.,Hahn, Q.,Nowak, R.P.,Viennet, T.,Orellana, E.A.,Roy Burman, S.S.,Yue, H.,Hunkeler, M.,Fontana, P.,Wu, H.,Arthanari, H.,Fischer, E.S.,Gregory, R.I.
Structural basis of regulated m 7 G tRNA modification by METTL1-WDR4.
Nature, 613:391-397, 2023

PubMed Abstract: Chemical modifications of RNA have key roles in many biological processes. N-methylguanosine (mG) is required for integrity and stability of a large subset of tRNAs. The methyltransferase 1-WD repeat-containing protein 4 (METTL1-WDR4) complex is the methyltransferase that modifies G46 in the variable loop of certain tRNAs, and its dysregulation drives tumorigenesis in numerous cancer types. Mutations in WDR4 cause human developmental phenotypes including microcephaly. How METTL1-WDR4 modifies tRNA substrates and is regulated remains elusive. Here we show,  through structural, biochemical and cellular studies of human METTL1-WDR4, that WDR4 serves as a scaffold for METTL1 and the tRNA T-arm. Upon tRNA binding, the αC region of METTL1 transforms into a helix, which together with the α6 helix secures both ends of the tRNA variable loop. Unexpectedly, we find that the predicted disordered N-terminal region of METTL1 is part of the catalytic pocket and essential for methyltransferase activity. Furthermore, we reveal that S27 phosphorylation in the METTL1 N-terminal region inhibits methyltransferase activity by locally disrupting the catalytic centre. Our results provide a molecular understanding of tRNA substrate recognition and phosphorylation-mediated regulation of METTL1-WDR4, and reveal the presumed disordered N-terminal region of METTL1 as a nexus of methyltransferase activity.

PubMed: 36599985
DOI: 10.1038/s41586-022-05566-4

Experimental method

X-RAY DIFFRACTION (3.1 Å)