7U20
Crystal structure of human METTL1 and WDR4 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-02 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 124.407, 194.574, 86.346 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.870 - 3.100 |
R-factor | 0.224 |
Rwork | 0.222 |
R-free | 0.27080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3CKK for METTL1 chain D of 2VDU for WDR4 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.435 |
Data reduction software | XDS (VERSION Jan 31, 2020 BUILT=20200131) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | BUSTER (2.10.4) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.870 | 47.870 | 3.310 |
High resolution limit [Å] | 3.090 | 8.750 | 3.090 |
Rmerge | 0.308 | 0.039 | 2.649 |
Rmeas | 0.377 | 0.048 | 3.236 |
Rpim | 0.214 | 0.028 | 1.831 |
Number of reflections | 19262 | 892 | 3381 |
<I/σ(I)> | 5.1 | 20.8 | 0.9 |
Completeness [%] | 99.0 | 96.4 | 97.5 |
Redundancy | 4.6 | 4.5 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 2M (NH4)2SO4, 0.2M K Na Tartrate, 0.1 M Na3 citrate pH 6.5 |