7U0F

HIV-1 Rev in complex with tubulin

Summary for 7U0F

• Entry DOI: 10.2210/pdb7u0f/pdb
• EMDB information: 26257
• Descriptor: Tubulin alpha-1A chain, Tubulin beta chain, Protein Rev (3 entities in total)
• Functional Keywords: rev, tubulin, hiv-1, viral protein
• Biological source: Human immunodeficiency virus 1; More
• Total number of polymer chains: 10
• Total formula weight: 278392.19

Authors

Eren, E. (deposition date: 2022-02-18, release date: 2023-08-23, Last modification date: 2023-10-18)

Primary citation

Eren, E.,Watts, N.R.,Randazzo, D.,Palmer, I.,Sackett, D.L.,Wingfield, P.T.
Structural basis of microtubule depolymerization by the kinesin-like activity of HIV-1 Rev.
Structure, 31:1233-, 2023

PubMed Abstract: HIV-1 Rev is an essential regulatory protein that transports unspliced and partially spliced viral mRNAs from the nucleus to the cytoplasm for the expression of viral structural proteins. During its nucleocytoplasmic shuttling, Rev interacts with several host proteins to use the cellular machinery for the advantage of the virus. Here, we report the 3.5 Å cryo-EM structure of a 4.8 MDa Rev-tubulin ring complex. Our structure shows that Rev's arginine-rich motif (ARM) binds to both the acidic surfaces and the C-terminal tails of α/β-tubulin. The Rev-tubulin interaction is functionally homologous to that of kinesin-13, potently destabilizing microtubules at sub-stoichiometric levels. Expression of Rev in astrocytes and HeLa cells shows that it can modulate the microtubule cytoskeleton within the cellular environment. These results show a previously undefined regulatory role of Rev.

PubMed: 37572662
DOI: 10.1016/j.str.2023.07.009

Experimental method

ELECTRON MICROSCOPY (3.53 Å)