7TFS
Cryo-EM of the OmcE nanowires from Geobacter sulfurreducens
Summary for 7TFS
| Entry DOI | 10.2210/pdb7tfs/pdb |
| EMDB information | 25879 |
| Descriptor | Cytochrome c, HEME C (2 entities in total) |
| Functional Keywords | helical symmetry, cytochrome nanowire, filament, electron transport, protein fibril |
| Biological source | Geobacter sulfurreducens |
| Total number of polymer chains | 1 |
| Total formula weight | 26313.75 |
| Authors | Wang, F.,Mustafa, K.,Chan, C.H.,Joshi, K.,Hochbaum, A.I.,Bond, D.R.,Egelman, E.H. (deposition date: 2022-01-07, release date: 2022-05-04, Last modification date: 2025-05-14) |
| Primary citation | Wang, F.,Mustafa, K.,Suciu, V.,Joshi, K.,Chan, C.H.,Choi, S.,Su, Z.,Si, D.,Hochbaum, A.I.,Egelman, E.H.,Bond, D.R. Cryo-EM structure of an extracellular Geobacter OmcE cytochrome filament reveals tetrahaem packing. Nat Microbiol, 7:1291-1300, 2022 Cited by PubMed Abstract: Electrically conductive appendages from the anaerobic bacterium Geobacter sulfurreducens were first observed two decades ago, with genetic and biochemical data suggesting that conductive fibres were type IV pili. Recently, an extracellular conductive filament of G. sulfurreducens was found to contain polymerized c-type cytochrome OmcS subunits, not pilin subunits. Here we report that G. sulfurreducens also produces a second, thinner appendage comprised of cytochrome OmcE subunits and solve its structure using cryo-electron microscopy at ~4.3 Å resolution. Although OmcE and OmcS subunits have no overall sequence or structural similarities, upon polymerization both form filaments that share a conserved haem packing arrangement in which haems are coordinated by histidines in adjacent subunits. Unlike OmcS filaments, OmcE filaments are highly glycosylated. In extracellular fractions from G. sulfurreducens, we detected type IV pili comprising PilA-N and -C chains, along with abundant B-DNA. OmcE is the second cytochrome filament to be characterized using structural and biophysical methods. We propose that there is a broad class of conductive bacterial appendages with conserved haem packing (rather than sequence homology) that enable long-distance electron transport to chemicals or other microbial cells. PubMed: 35798889DOI: 10.1038/s41564-022-01159-z PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.3 Å) |
Structure validation
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