7TD3

Sphingosine-1-phosphate receptor 1-Gi complex bound to S1P

7TD3 の概要

• エントリーDOI: 10.2210/pdb7td3/pdb
• EMDBエントリー: 25822
• 分子名称: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(i) subunit alpha-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, ... (6 entities in total)
• 機能のキーワード: gpcr, complex, lipid, membrane protein
• 由来する生物種: Bos taurus (cattle); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 132964.49

構造登録者

Liu, S.,Paknejad, N.,Zhu, L.,Kihara, Y.,Ray, D.,Chun, J.,Liu, W.,Hite, R.K.,Huang, X.Y. (登録日: 2021-12-30, 公開日: 2022-02-09, 最終更新日: 2024-10-09)

主引用文献

Liu, S.,Paknejad, N.,Zhu, L.,Kihara, Y.,Ray, M.,Chun, J.,Liu, W.,Hite, R.K.,Huang, X.Y.
Differential activation mechanisms of lipid GPCRs by lysophosphatidic acid and sphingosine 1-phosphate.
Nat Commun, 13:731-731, 2022

PubMed Abstract: Lysophospholipids are bioactive lipids and can signal through G-protein-coupled receptors (GPCRs). The best studied lysophospholipids are lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P). The mechanisms of lysophospholipid recognition by an active GPCR, and the activations of lysophospholipid GPCR-G-protein complexes remain unclear. Here we report single-particle cryo-EM structures of human S1P receptor 1 (S1P) and heterotrimeric G complexes formed with bound S1P or the multiple sclerosis (MS) treatment drug Siponimod, as well as human LPA receptor 1 (LPA) and G complexes in the presence of LPA. Our structural and functional data provide insights into how LPA and S1P adopt different conformations to interact with their cognate GPCRs, the selectivity of the homologous lipid GPCRs for S1P versus LPA, and the different activation mechanisms of these GPCRs by LPA and S1P. Our studies also reveal specific optimization strategies to improve the MS-treating S1P-targeting drugs.

PubMed: 35136060
DOI: 10.1038/s41467-022-28417-2

実験手法

ELECTRON MICROSCOPY (3 Å)