7T8W

Structure of antibody 3G12 bound to Respiratory Syncytial Virus G central conserved domain mutant S177Q

7T8W の概要

• エントリーDOI: 10.2210/pdb7t8w/pdb
• 分子名称: 3G12 Fab Light chain, 3G12 Fab Heavy chain, Mature secreted glycoprotein G (3 entities in total)
• 機能のキーワード: respiratory syncytial virus, human antibody, viral protein, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 53834.27

構造登録者

Nunez Castrejon, A.M.,O'Rourke, S.M.,Kauvar, L.M.,DuBois, R.M. (登録日: 2021-12-17, 公開日: 2022-02-09, 最終更新日: 2024-10-30)

主引用文献

Nunez Castrejon, A.M.,O'Rourke, S.M.,Kauvar, L.M.,DuBois, R.M.
Structure-Based Design and Antigenic Validation of Respiratory Syncytial Virus G Immunogens.
J.Virol., 96:e0220121-e0220121, 2022

PubMed Abstract: Respiratory syncytial virus (RSV) is a leading cause of severe lower respiratory tract disease of children, the elderly, and immunocompromised individuals. Currently, there are no FDA-approved RSV vaccines. The RSV G glycoprotein is used for viral attachment to host cells and impairment of host immunity by interacting with the human chemokine receptor CX3CR1. Antibodies that disrupt this interaction are protective against infection and disease. Nevertheless, development of an RSV G vaccine antigen has been hindered by its low immunogenicity and safety concerns. A previous study described three engineered RSV G proteins containing single-point mutations that induce higher levels of IgG antibodies and have improved safety profiles compared to wild-type RSV G (H. C. Bergeron, J. Murray, A. M. Nuñez Castrejon, et al., Viruses 13:352, 2021, https://doi.org/10.3390/v13020352). However, it is unclear if the mutations affect RSV G protein folding and display of its conformational epitopes. In this study, we show that the RSV G S177Q protein retains high-affinity binding to protective human and mouse monoclonal antibodies and has equal reactivity as wild-type RSV G protein to human reference immunoglobulin to RSV. Additionally, we determined the high-resolution crystal structure of RSV G S177Q protein in complex with the anti-RSV G antibody 3G12, further validating its antigenic structure. These studies show for the first time that an engineered RSV G protein with increased immunogenicity and safety retains conformational epitopes to high-affinity protective antibodies, supporting its further development as an RSV vaccine immunogen. Respiratory syncytial virus (RSV) causes severe lower respiratory diseases of children, the elderly, and immunocompromised populations. There currently are no FDA-approved RSV vaccines. Most vaccine development efforts have focused on the RSV F protein, and the field has generally overlooked the receptor-binding antigen RSV G due to its poor immunogenicity and safety concerns. However, single-point mutant RSV G proteins have been previously identified that have increased immunogenicity and safety. In this study, we investigate the antibody reactivities of three known RSV G mutant proteins. We show that one mutant RSV G protein retains high-affinity binding to protective monoclonal antibodies, is equally recognized by anti-RSV antibodies in human sera, and forms the same three-dimensional structure as the wild-type RSV G protein. Our study validates the structure-guided design of the RSV G protein as an RSV vaccine antigen.

PubMed: 35266806
DOI: 10.1128/jvi.02201-21

実験手法

X-RAY DIFFRACTION (3.1 Å)