7SXL

Plasmodium falciparum apicoplast DNA polymerase (exo-minus) without affinity tag

Summary for 7SXL

• Entry DOI: 10.2210/pdb7sxl/pdb
• Related: 5DKU
• Descriptor: Plastid replication-repair enzyme, GLYCEROL, SODIUM ION, ... (7 entities in total)
• Functional Keywords: dna polymerase, exonulease, apicoplast, replication, transferase
• Biological source: Plasmodium falciparum (isolate 3D7)
• Total number of polymer chains: 2
• Total formula weight: 149280.43

Authors

Nieto, N.,Chheda, P.,Kerns, R.,Nelson, S.,Honzatko, R. (deposition date: 2021-11-23, release date: 2022-10-26, Last modification date: 2023-10-18)

Primary citation

Chheda, P.R.,Nieto, N.,Kaur, S.,Beck, J.M.,Beck, J.R.,Honzatko, R.,Kerns, R.J.,Nelson, S.W.
Promising antimalarials targeting apicoplast DNA polymerase from Plasmodium falciparum.
Eur.J.Med.Chem., 243:114751-114751, 2022

PubMed Abstract: Malaria is caused by the parasite Plasmodium falciparum, which contains an essential non-photosynthetic plastid called the apicoplast. A single DNA polymerase, apPOL, is targeted to the apicoplast, where it replicates and repairs the genome. apPOL has no direct orthologs in mammals and is considered a promising drug target for the treatment and/or prevention of malaria. We previously reported screening the Malaria Box to identify MMV666123 as an inhibitor of apPOL. Herein we extend our studies and report structure-activity relationships for MMV666123 and identify key structural motifs necessary for inhibition. Although attempts to crystallize apPOL with the inhibitor were not fruitful, kinetic analysis and crystal structure determinations of WT and mutant apo-enzymes, facilitated model building and provided insights into the putative inhibitor binding site. Our results validate apPOL as an antimalarial target and provide an avenue for the design of high potency, specific inhibitors of apPOL and other A-family DNA polymerases.

PubMed: 36191407
DOI: 10.1016/j.ejmech.2022.114751

Experimental method

X-RAY DIFFRACTION (2.7 Å)