7SXL

Plasmodium falciparum apicoplast DNA polymerase (exo-minus) without affinity tag

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 65
Unit cell lengths	145.369, 145.369, 164.944
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.910 - 2.700
R-factor	0.1918
Rwork	0.191
R-free	0.22370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dku
RMSD bond length	0.004
RMSD bond angle	0.552
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.910	2.750
High resolution limit [Å]	2.700	2.700
Number of reflections	54200	2312
<I/σ(I)>	0.8
Completeness [%]	100.0
Redundancy	6.7
CC(1/2)	0.990	0.539

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Equal parts protein and precipitant solutions. Protein solution: 20 mg/ml protein, 20 mM Tris, pH 8.0, 500 mM NaCl, 10% glycerol and 5 mM imidazole. Precipitant solution: PEG 1000 30% (v/v), 100 sodium citrate tribasic dihydrate, pH 5.5, 400 mM NaCl.