7S3H
Cas9:sgRNA:DNA (S. pyogenes) with 0 RNA:DNA base pairs, open-protein/linear-DNA conformation
Summary for 7S3H
Entry DOI | 10.2210/pdb7s3h/pdb |
Related | 7S36 7S37 7S38 |
EMDB information | 24817 24818 24819 24823 |
Descriptor | Non-target DNA strand, CRISPR-associated endonuclease Cas9/Csn1, Single-guide RNA, ... (4 entities in total) |
Functional Keywords | dnase, complex, ribonucleoprotein, genome editor, hydrolase-rna-dna complex, hydrolase/rna/dna |
Biological source | Streptococcus pyogenes serotype M1 More |
Total number of polymer chains | 4 |
Total formula weight | 210266.52 |
Authors | Cofsky, J.C.,Soczek, K.M.,Knott, G.J.,Nogales, E.,Doudna, J.A. (deposition date: 2021-09-06, release date: 2022-04-20, Last modification date: 2022-05-04) |
Primary citation | Cofsky, J.C.,Soczek, K.M.,Knott, G.J.,Nogales, E.,Doudna, J.A. CRISPR-Cas9 bends and twists DNA to read its sequence. Nat.Struct.Mol.Biol., 29:395-402, 2022 Cited by PubMed: 35422516DOI: 10.1038/s41594-022-00756-0 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (2.5 Å) |
Structure validation
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